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C1154

Sigma-Aldrich

4-Chloro-7-sulfobenzofurazan ammonium salt

Powder

Synonym(s):

4-Chloro-7-sulfobenz-2-oxa-1,3-diazole ammonium salt, 7-Chloro-2,1,3-benzoxadiazole-4-sulfonic acid ammonium salt, 7-Chloro-4-benzofurazansulfonic acid ammonium salt, SBF-Chloride

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About This Item

Linear Formula:
C6H3ClN2O4S · NH3
CAS Number:
81377-14-2
Molecular Weight:
251.65
Beilstein/REAXYS Number:
5200152
MDL number:
MFCD00038995
UNSPSC Code:
12171500
PubChem Substance ID:
24892366
NACRES:
NA.47

Product Name

4-Chloro-7-sulfobenzofurazan ammonium salt,

assay

>98%

Quality Level

200

form

powder

color

brown
light yellow

mp

>300 °C (lit.)

solubility

water: 50 mg/mL, clear, dark yellow

fluorescence

λex 360 nm; λem 410 nm (pH 8.5)
λex 383 nm; λem 528 nm (Reaction product)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

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Related Categories

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
103F05521

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R Sutton et al.
European journal of biochemistry, 142(2), 387-392 (1984-07-16)
Bovine heart mitochondrial ATPase is inhibited after covalent modification with 4-chloro-7-nitrobenzofuroxan. The kinetics of the reaction are indistinguishable from those for the reaction of an essential tyrosine residue of the ATPase with 4-chloro-7-nitrobenzofurazan that have been described previously [Ferguson et
X P Chen et al.
Journal of chromatography. B, Biomedical sciences and applications, 709(1), 19-25 (1998-07-08)
Fluorescent adducts of 4-chloro-7-sulphobenzofurazan with cysteine, cysteinylglycine, reduced glutathione and N-acetylcysteine were prepared. Adducts were separated by HPLC on a 3-mm Nova-Pak C18 reversed-phase column using isocratic elution with a solvent of acetonitrile-0.15 M phosphoric acid (5:95) buffered at pH
Ammonium 4-chloro-7-sulfobenzofurazan: a new fluorigenic thiol-specific reagent.
J L Andrews et al.
Archives of biochemistry and biophysics, 214(1), 386-396 (1982-03-01)
R M Bolton et al.
Analytical biochemistry, 216(2), 418-423 (1994-02-01)
Ammonium 4-chloro-7-sulfobenzofurazan (Sbf-Cl) is a water-soluble fluorescent reagent which is highly specific for thiol groups. We describe here a simple and sensitive fluorescence assay which is highly specific for subunit 3 of the rat glutathione S-transferases. Specific activities of isoenzymes
Bin Meng et al.
BioMed research international, 2015, 815210-815210 (2015-11-12)
This study was aimed at investigating the effects of quercetin on mRNA expression and activity of critical enzymes in homocysteine metabolism in rats fed a methionine-enriched diet. Rats were fed for 6 weeks the following diets, that is, control, 0.5%

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