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C-12914

Sigma-Aldrich

Human M1 Macrophages (hMDM-GMCSF(-))

Monocyte-derived, single donor, 1.5 million cryopreserved cells

Synonym(s):

hMDM-GMCSF(-) cells

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10 MG
CA$106.00
50 MG
CA$398.00

CA$106.00


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10 MG
CA$106.00
50 MG
CA$398.00

About This Item

UNSPSC Code:
41106514
NACRES:
NA.81

CA$106.00


Please contact Customer Service for Availability

Request a Bulk Order
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biological source

human blood (monocyte)

packaging

pkg of 1,500,000 cells

morphology

(macrophages)

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

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This Item
WHA2630990WHA2626990WHA2628990
detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

feature

pH Indicators, Integral Comparison Strip, 1.0 to 12.0 range

feature

pH Indicators, Integral Comparison Strip, 8.0 to 9.7 range

feature

pH Indicators, Integral Comparison Strip, 1.8 to 3.8 range

feature

pH Indicators, Integral Comparison Strip, 5.2 to 6.8 range

packaging

pkg of 200 ea

packaging

pkg of 200 ea

packaging

pkg of 200 ea

packaging

pkg of 200 ea

manufacturer/tradename

Whatman 2612-990

manufacturer/tradename

Whatman 2630-990, Whatman Article No. 28418930 (US reference)

manufacturer/tradename

Whatman 2626-990, Whatman Article No. 28418926 (US reference)

manufacturer/tradename

Whatman 2628-990, Whatman Article No. 28418928 (US reference)

W × L

11 mm × 100 mm

W × L

11 mm × 100 mm

W × L

11 mm × 100 mm

W × L

11 mm × 100 mm

General description

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

Cell Line Origin

Monocyte

Application

Cryopreserved human monocyte-derived macrophages (hMDM) are convenient and easy-to-handle. The thawed cells plate into all tissue culture vessel formats and can be maintained as adherent, biologically functional cultures for several weeks. Optionally, user-customizable activation of the cells can be performed.Cryopreserved human macrophages are produced from monocytes in M1 Macrophage Generation Media DXF and are available as non-activated, fully qualified M1- (GM-CSF) polarized cells. After 9-10 days of differentiation, the M1 macrophages are cryopreserved using Cryo-SFM. Each cryo vial contains more than 1.5 million viable cells after thawing.

Warning

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Subculture Routine

Click here for more information.

Other Notes

Recommended Plating Density: 100000 cells per cm2Tested Markers: CD80 positive, CD68 positiveNote: The plated cells do not proliferate. Recommended plating density for obtaining an adherent cell monolayer with 70-90% confluency.

Recommended products

Recommended Primary Cell Culture Media:Link
Recommended Primary Cell Culture Media:Link

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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    PD n-3 DPA Pathway Regulates Human Monocyte Differentiation and Macrophage Function
    Pistorius K, et al.
    Cell Chemical Biology, 25(6), 749-760 (2018)
    You-Yang Qu et al.
    Neurochemical research, 40(1), 1-14 (2014-11-05)
    Epoxyeicosatrienoic acids (EETs), the cytochrome P450 epoxygenase metabolite of arachidonic acid, have been demonstrated to have neuroprotective effect. Phosphatidylinositol 3-kinase (PI3K)/Akt and ATP-sensitive potassium (KATP) channels are thought to be important factors that mediate neuroprotection. However, little is known about
    Soluble epoxide hydrolase inhibitor, 12-(3-adamantan-1-yl-ureido)-dodecanoic acid, represses human aortic smooth muscle cell proliferation and migration by regulating cell death pathways via the mTOR signaling
    Li SH, et al.
    International Journal of Clinical and Experimental Pathology, 10(8), 8434-8442 (2017)
    Aylin Acun et al.
    Acta biomaterialia, 94, 372-391 (2019-05-31)
    Deaths attributed to ischemic heart disease increased by 41.7% from 1990 to 2013. This is primarily due to an increase in the aged population, however, research on cardiovascular disease (CVD) has been overlooking aging, a well-documented contributor to CVD. The
    Jing Li et al.
    Frontiers in bioscience : a journal and virtual library, 13, 3480-3487 (2008-05-30)
    In stroke-prone spontaneously hypertensive rats (SHRSP) end-organ damage is markedly accelerated by high-salt (HS) intake. Since epoxyeicosatrienoic acids (EETs) possess vasodepressor and natriuretic activities, we examined whether a soluble epoxide hydrolase (sEH) inhibitor, 12-(3-adamantan-1-yl-ureido)-dodecanoic acid (AUDA), to inhibit the metabolism

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