C1427

Cytochrome b₅ human

recombinant, expressed in E. coli (histidine-tagged), ≥90% (SDS-PAGE)

• Synonym(s): Cytochrome b5
• MDL number: MFCD02687283
• UNSPSC Code: 12161501
• NACRES: NA.47

Properties

• biological source: human
• Quality Level: 200
• recombinant: expressed in E. coli (histidine-tagged)
• assay: ≥90% (SDS-PAGE)
• form: solution
• mol wt: 16 kDa
• technique(s): inhibition assay: suitable
• suitability: suitable for molecular biology
• UniProt accession no.: P00167
• shipped in: dry ice

Description

General description

Cytochrome b5 (CYB5A) gene codes for cytochrome b5 protein. It is located on human chromosome 18. It is co-expressed in the Leydig cells of the testis and theca cells of the ovary.

Biochem/physiol Actions

Cytochrome b5 (CYB5A) mediates NADH-dependent reduction of methemoglobin (erythrocytes), electron transfer to fatty-acid desaturases (mainly liver) and steroid and xenobiotics metabolism by cytochrome P450 enzymes. It is essential for androstenedione synthesis. CYB5A helps in the efficient synthesis of gonadal androgen.

Cytochrome b₅ is a membrane-bound heme-containing protein that enhances the catalytic efficiency of some P450 isoforms. Its mechanism of action is unknown.

Membrane-bound heme-containing protein; enhances catalytic efficiency of some P450 isoforms.

Physical form

Solution in 20 mM phosphate buffer, pH 7.25, with 0.1 mM EDTA

Analysis Note

Heme content measured using difference spectroscopy. An extinction coefficient of 100 mM^-1 for the absorbance difference between 490 nm and 424 nm is used to calculate the concentration of cytochrome b₅.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)