C6144

Chlorophyll a

Powder

• Synonym(s): Pheophytin; universal photosynthetic pigment
• Empirical Formula (Hill Notation): C₅₅H₇₂MgN₄O₅
• CAS Number: 479-61-8
• Molecular Weight: 893.49
• Beilstein/REAXYS Number: 3586237
• EC Number: 207-536-6
• MDL number: MFCD00079050
• UNSPSC Code: 12171500
• PubChem Substance ID: 24892839
• NACRES: NA.47

Properties

• Product Name: Chlorophyll a, from Anacystis nidulans algae
• biological source: Anacystis nidulans algae
• Quality Level: 200
• form: powder
• technique(s): titration: suitable
• impurities: chlorophyll b, free
• color: blue to very dark blue
• mp: 150-153 °C
• solubility: diethyl ether: 0.1 mg/10 mL
• ε (extinction coefficient): 11.1-12.1 × 10⁴ at 428-430 nm in diethyl ether; 8.3-9.4 × 10⁴ at 660-662 nm in diethyl ether

Description

Application

A procedure has been described for harvesting native light-harvesting complex IIb (LHCIIb).

Chlorophyll a has been used as a standard for the calibration of fluorometer.[1][2]

Biochem/physiol Actions

Chlorophyll a is an essential pigment in oxygenic photosynthesis. In photosystems, it is present as an antenna pigment as well as in reaction center I and II.[3] Chlorophyll a is a blue-green pigment which absorbs light in 400-450nm and 640-660nm range. In photosynthesis, the energy absorbed by chlorophyll transforms carbon dioxide and water into carbohydrates and oxygen. When chlorophyll absorbs light energy, an electron in chlorophyll is excited from a lower energy state to a higher energy state. In this higher energy state, the electron is more readily transferred to another molecule. This starts a chain of electron-transfer steps, which ends with an electron transferred to carbon dioxide.[4]

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Sealed ampule.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)