C7867

Anti-Claspin (C-terminal) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• MDL number: MFCD06411798
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 250 kDa (170 kDa band that may correspond to a claspin related protein is observed in some cell lines)
• species reactivity: human
• technique(s): microarray: suitable; western blot: 1:250-1:500 using K562 cell extracts
• UniProt accession no.: Q9HAW4
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... CLSPN(63967)

Description

Immunogen

synthetic peptide corresponding to amino acids 1321-1339 of human claspin, conjugated to KLH via an N-terminal added cysteine residue.

Application

Anti-Claspin (C-terminal) antibody is suitable for microarray and western blot analysis at a dilution of 1:250-1:500.

Biochem/physiol Actions

Claspin is a protein which plays a major role in DNA replication. It involves in protecting DNA replication forks against DNA damage. In DNA damage response, claspin directly interacts with Rad17, which is required for ATR activation. ATR is activated by various DNA damage and DNA replication stress. Once activated, ATR (ataxia telangiectasia mutated-Rad3 related protein) phosphorylates effector kinase Chk1 which further depends on mediator protein claspin. Claspin helps to stimulate phosphorylation reaction of Chk1.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)