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C9830

Chitosanase from Streptomyces griseus

Name: Chitosanase from <I>Streptomyces griseus</I>
Brand: SIGMA

lyophilized powder, >50 units/mg protein (Bradford)

Synonym(s):

Chitosan N-acetylglucosaminohydrolase

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About This Item

CAS Number:

51570-20-8

EC Number:

3.2.1.132 (BRENDA, IUBMB)

MDL number:

MFCD00211797

UNSPSC Code:

12352204

NACRES:

NA.54

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form

lyophilized powder

Quality Level

200

specific activity

>50 units/mg protein (Bradford)

purified by

chromatography

solubility

H₂O: soluble, clear to slightly hazy, colorless (10U/0.3mL)

foreign activity

chitinase <1.0 units/mg protein

storage temp.

−20°C

Related Categories

Glycobiology Profiling Tools

Proteins & Enzymes

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This Item	EHU152321	EHU158371	EHU121211
EHU147601 MISSION^® esiRNA	EHU152321 MISSION^® esiRNA	EHU158371 MISSION^® esiRNA	EHU121211 MISSION^® esiRNA
esiRNA cDNA target sequence CCAGGGAGCCATCTACACTGTGGAGTACGCCTGCAGCGCCGTGAAGAACCTGGTGGACAGCAGCGTCTACTTCCGCAGCGTGGAGGGTCTGCTCAAACAGGCCATCAGCATCCGGGACCATATGAATGCCAGTGCCCAGGGCCACAGCCCGGAGGAACCACCCCCGCCCTCCTCAGCCTGATCCTGGAAGAGACTCGGGGCCCCCCAGCCTCCGCCAACCCAGACAAAGATCATTCCACTCAGCCTGGGACGATGGGGAGGAAAAAAATCCAGATCTCCCGCATCCTGGACCAAAGGAATCGGCAGGTGACGTTCACCAAGCGGAAGTTCGGGCTGATGAAGAA	esiRNA cDNA target sequence GCTATGCCTTGGGATCAAGATCCAGAACAATCAACTGGAAATTACAGTGAAGATGAACAAAATGGAAAGCAGAAATGGAGAGAAGAAGGAGAAGCAGGCAGAAAGAGAGAACGAGAAAAAGAAGAAAAAAACGAAAAGGAGCTGCAAGATGAACAGGAAAACAAAAGGAAAAGGGAAAATGAGAAACAGAAACAGTATCCCGAGAAAAGATTAGTCAGCAAATCCCTCATGCATACTCTCTGGGCAAAGTTTAAGTTAAACAGGTGCCCCACTATACAAGAGAGTCTATCACTGTCATTTGAATTTGACATGACACATAAACAGATAAGTCAATGGTTTTGTAAAACGAGGAAGAAATATAATAAAGAAATGTCCAAGAGAAAGCATAAGAAAAAACATATGAGATGGAGATCTCTGTGTTGCCAAGGCT	esiRNA cDNA target sequence CTCCATCCCCTAGTTGCAGACTCATCACCATGGTTACCATAGTGACTGCTTCATTGCCATGGTTACATACTAATTGCTGCAGCTCTGTGGCCCAGCCCACTGCTTCAGCTGTGGGCCATCTGAGGGTACGTGCCATCATCTCTCCAGCCCAGGCCCCTGGGCATCTCATGCTGGGGGGAAGGGACTGAATACCTTTTTCCTTCCCCCTGCCTGTGTCTTCAGCCCTGATGCACAGGCTGCCAGCCCCCCAGTCCAGCCCTCTCCCTTCCACTGGTGCCTTGCTTAGAGCCAGAAGGGATGAAGCCGGGGGATCTATGGAACAGAGGAGGAGCGATGCAGTTGGGAGAGGAAGCTAGAAGGGTTATGGTTGGAGTTCTGTACAGTGTTGAGTTTCCGACAGGGAA	esiRNA cDNA target sequence CTCTCCCTGGAGGCCTATCTCCCTCAGAACTGCGCGAGAAGCGAGACCTTAGAAGGCAGGGCTTCCCGCGAAGGACCGGAAAGGAGCGCCTACTAAGGACGCCGTCGAGGTCCGGGGCGCCTCAACTCTATAGCTCTAACTGGCTAGAAGTGCCCAACGTGGAATGTTTCTTTTTTAAAGGCGGCTCTTGAAGCGACCCGGAAGCGGAAGTGGAAGAAAGTTCTAGTGGCTTGAGGTATCCGCAGGAGCGGCCGGGTGGCGGGAGGAACCGTTACGGGAACTGAAGTTGCGGATTAAGCCTGATCAAGATGACAACCTCCCAAAAGCACCGAGACTTCGTGGCAGAGCCCATGGGGGAGAAGCCAGTGGGGAGCCTGGCTGGGATTGGTGAAGTCCTGGGCAAGAAG
product line MISSION^®	product line MISSION^®	product line MISSION^®	product line MISSION^®
Gene Information human ... MEF2B(4207)	Gene Information human ... NANOGNB(360030)	Gene Information human ... TREH(11181), PHLDB1(23187)	Gene Information human ... BANF1(8815)
Ensembl \| human accession no. ENSG00000064489	Ensembl \| human accession no. ENSG00000205857	Ensembl \| human accession no. ENSG00000019144	Ensembl \| human accession no. ENSG00000175334
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
NCBI accession no. NM_001145785	NCBI accession no. NM_001145465	NCBI accession no. NM_001144758	NCBI accession no. NM_001143985

General description

Chitosanase is synthesized from Streptomyces griseus as an extracellular secreted enzyme and corresponds to a molecular weight of 34 kDa.[1] It has a temperature and pH optimum of 60°C and 5.7, respectively. Chitosanase belongs to the glycosyl hydrolase family[1] and comprises α-helical tertiary structure similar to lysozyme with substrate-binding cleft placed between two lobes.[2]

Application

Chitosanase from Streptomyces griseus has been used in a study to assess the effect of chitin sources on production of chitinase and chitosanase. Chitosanase from Streptomyces griseus has also been used in a study to investigate the effective production of chitinase and chitosanase by Streptomyces griseus HUT 6037 using colloidal chitin and various degrees of deacetylation of chitosan.

Chitosanase from Streptomyces griseus has been used in the digestion of lacrimal gland (LG) tissues.[3]

The enzyme from Sigma has been used for the hydrolysis of the crude cell wall preparation of oomycete Aphanomyces euteiches, a major parasite of legume plants.[4] It has also been used for the enzymatic hydrolysis of the fully de-N-acetylated chitosan to get chitosan oligomer mixtures during the preparation of biocompatible chitosan-alginate gel.[5]

Biochem/physiol Actions

Chitosanase catalyzes the endohydrolysis of N-acetylated chitosan specifically at the of β(1,4) linkages between N-acetyl-D-glucosamine and D-glucosamine residues. Chitosanase from Streptomyces griseus is capable of hydrolyzing both chitosan and carboxymethyl cellulose. Chitosanase is found in several types of microorganisms[6] and is used for the lysis of fungal cell walls.[2]

Unit Definition

One unit will release 1 μmole of glucosamine from chitosan per min at pH 5.0 at 37 °C as measured in the fluorimetric assay of Osswald, et al. (1992).

Physical form

Lyophilized powder containing potassium phosphate buffer salts

Preparation Note

For long term solution storage, reconstitute vial with 50% glycerol solution. Dilute to working concentrations with a 0.1% BSA solution.

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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