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C9993

Sigma-Aldrich

Anti-CD9 antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-BA2, Anti-BTCC-1, Anti-DRAP-27, Anti-GIG2, Anti-Growth-inhibiting gene 2 protein, Anti-Leukocyte antigen MIC3, Anti-MRP-1, Anti-Motility related protein, Anti-TSPAN29, Anti-p24 antigen

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About This Item

MDL number:
MFCD00164638
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~25 kDa

species reactivity

human, mouse, rat

concentration

~1 mg/mL

technique(s)

immunohistochemistry: 1:100-1:250
western blot: 1:500-1:1,000

General description

CD9 is a glioma stem cell (GSC)-enriched protein, which belongs to tetraspanin family. It is a cell motility related molecule. The gene is located on human chromosome 12p13.31. CD9 is expressed on pre-B cells, activated T cells and Schwann cells.

Immunogen

synthetic peptide corresponding to residues 36-50 of human CD9.

Application

Anti-CD9 antibody produced in rabbit has been used in
  • immunostaining
  • identification of hypoxia-induced mesenchymal stem cells (MSC-MVs)
Anti-CD9 antibody produced in rabbit is suitable for immunohistochemistry at a working dilution of 1:100-1:250 and western blotting at a working dilution of 1:500-1:1000. It was used as a primary antibody for the immunolocalization of adhesion protein CD9 during a study to investigate the impact of adhesion proteins on skeletal myoblast differentiation.

Biochem/physiol Actions

CD9 plays a major role in sperm-egg fusion in fertilization. It enhances cancer invasion and tumor growth in cervical cancer, gastric cancer and glioblastoma (GBM). CD9 is involved in cell invasion, apoptosis and resistance to chemotherapy.

Target description

The CD9 protein is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily proteins. It can modulate cell adhesion and migration. It also triggers platelet activation and aggregation. In addition, the protein appears to promote muscle cell fusion and support myotube maintenance.

Physical form

solution in phosphate buffered saline, containing 0.02% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number
SLCC3420
SLBW3323
SLBT1734
SLBP0637V
SLBL5144V

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