4-11
11033103, human cervix, Epithelial
Synonym(s):
HeLa
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About This Item
UNSPSC Code:
41106514
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Product Name
4-11, 11033103
biological source
human cervix
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
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Cell Line Origin
HeLa, genetic modification, human androgen receptor, firefly luciferase reporter gene, MMTV promoter
Cell Line Description
4-11 is a HeLa cell line stably transfected with two constructs: the human androgen receptor (hAR) expression construct; a firefly luciferase reporter gene under the control of the MMTV promoter. 4-11 can be used to detect androgenic activity of chemicals.
DNA Profile
STR-PCR Data:
Amelogenin: X
CSF1PO: 9,10
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18
Amelogenin: X
CSF1PO: 9,10
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18
Culture Medium
DMEM (D6546) + 2 mM L-Glutamine (G7513) + 10% FBS / FCS (F2442) + 16 μg/ml Blasticidin S HCL + 800 μg/ml G418.
Subculture Routine
Split sub-confluent cultures (70-80%) 1:4 to 1:10, i.e. seeding at 2 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 °C.
Other Notes
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Legal Information
US7537904B1 N Ooe & Matsunaga H 2009.Cell for measuring the ability to control the activity of a ligand-responsive transcription control factor.
Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
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