SW 1116

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 87071006, human colon, Epithelial

• UNSPSC Code: 41106514

Properties

• biological source: human colon
• packaging: tube of 5 μg 87071006-DNA-5UG; pkg of vial of cells 87071006-1VL
• growth mode: Adherent
• karyotype: 2n = 46, hypotriploid
• morphology: Epithelial
• products: Carcinoembryonic antigen (CEA)
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human Caucasian colon adenocarcinoma

Cell Line Description

Derived from a grade II adenocarcinoma of the colon extending into the muscularis from a 73 year old Caucasian male. The cells exhibit brush borders and produce high levels of CEA.

Application

Tumourigenicity studies

Culture Medium

L15 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-3x10,000 cells/cm² using 0.25% trypsin/EDTA; CO₂ is not needed. Medium change after 4-5 days. 37°C.

Other Notes

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