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D2197

Sigma-Aldrich

Diaphorase from Clostridium kluyveri

recombinant, expressed in E. coli, Animal-component free

Synonym(s):

Diaphorase, Lipoamide Dehydrogenase, Lipoyl Dehydrogenase

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About This Item

EC Number:
1.8.1.4 (BRENDA, IUBMB)
UNSPSC Code:
12352204
NACRES:
NA.54
Pricing and availability is not currently available.

recombinant

expressed in E. coli

Quality Level

200

form

solid

specific activity

>=30 units/mg protein (biuret)

storage temp.

−20°C

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Whatman® Nuclepore™ Track-Etched Membranes

diam.

(19 mm)

diam.

(19 mm)

diam.

(19 mm)

diam.

(13 mm)

pore size

0.4 μm pore size

pore size

0.8 μm pore size

pore size

0.2 μm pore size

pore size

0.8 μm pore size

manufacturer/tradename

Cytiva, Whatman 10417104

manufacturer/tradename

Cytiva, Whatman 10417304

manufacturer/tradename

Whatman 10417004

manufacturer/tradename

Cytiva, Whatman 10417301

material

polycarbonate

material

polycarbonate

material

polycarbonate

material

polycarbonate

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

sterility

-

packaging

pack of 100 ea

packaging

pack of 100 ea

packaging

pack of 100 ea

packaging

pack of 100 ea

General description

Research area: Cancer

Application

Diaphorase from Clostridium kluyveri has been used:
  • in lactate dehydrogenase assay in gill cells.[1]
  • impregnated on the strip for formate dehydrogenase assay.[2]
  • in various assays to oxidate β-NADH or β-NADPH in the presence of an electron acceptor.
  • for semiquinone generation during benzoquinone reduction.[3]
  • for evaluation of glucose uptake in cultured muscle cells.[4]
  • in lactate dehydrogenase cytotoxicity assay.[5]

Biochem/physiol Actions

Diaphorase catalyzes the oxidation of either β-NADH or β-NADPH in the presence of an electron acceptor such as methylene blue or 2,6-dichlorophenolindophenol. Diaphorases that are specific for either β-NADH or β-NADPH are known. The pig heart enzyme of Straub seems to have native diaphorase (β-NADH specific) as well as lipoic and lipoamide dehydrogenase activities.

Unit Definition

One unit of diaphorase will oxidize 1.0 micromole of beta-NADH per minute at pH 7.5 at 25 degrees C, with the corresponding reduction of the appropriate electron acceptor.

Physical form

Supplied as a lyophilized powder containing trehalose.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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    Certificates of Analysis (COA)

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    SLBB5497V
    SLBB5497

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