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D2197

Sigma-Aldrich

Diaphorase from Clostridium kluyveri

recombinant, expressed in E. coli, Animal-component free

Synonym(s):

Diaphorase, Lipoamide Dehydrogenase, Lipoyl Dehydrogenase

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About This Item

EC Number:
1.8.1.4 (BRENDA, IUBMB)
UNSPSC Code:
12352204
NACRES:
NA.54
Pricing and availability is not currently available.

recombinant

expressed in E. coli

Quality Level

200

form

solid

specific activity

>=30 units/mg protein (biuret)

storage temp.

−20°C

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This Item
D554010171832001M1567
Diaphorase from Clostridium kluyveri recombinant, expressed in E. coli, Animal-component free

D2197

Diaphorase from Clostridium kluyveri

Diaphorase from Clostridium kluyveri lyophilized powder, 3.0-20.0 units/mg protein (biuret)

D5540

Diaphorase from Clostridium kluyveri

Aldehyde Dehydrogenase from yeast

10171832001

Aldehyde Dehydrogenase

Malic Dehydrogenase from porcine heart ≥600 units/mg protein (biuret), ammonium sulfate suspension

M1567

Malic Dehydrogenase from porcine heart

specific activity

>=30 units/mg protein (biuret)

specific activity

3.0-20.0 units/mg protein (biuret)

specific activity

~20 units/mg protein (At 25 °C with acetaldehyde as the substrate.)

specific activity

≥600 units/mg protein (biuret)

form

solid

form

lyophilized powder

form

lyophilized

form

ammonium sulfate suspension

recombinant

expressed in E. coli

recombinant

-

recombinant

-

recombinant

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

200

General description

Research area: Cancer

Application

Diaphorase from Clostridium kluyveri has been used:
  • in lactate dehydrogenase assay in gill cells.[1]
  • impregnated on the strip for formate dehydrogenase assay.[2]
  • in various assays to oxidate β-NADH or β-NADPH in the presence of an electron acceptor.
  • for semiquinone generation during benzoquinone reduction.[3]
  • for evaluation of glucose uptake in cultured muscle cells.[4]
  • in lactate dehydrogenase cytotoxicity assay.[5]

Biochem/physiol Actions

Diaphorase catalyzes the oxidation of either β-NADH or β-NADPH in the presence of an electron acceptor such as methylene blue or 2,6-dichlorophenolindophenol. Diaphorases that are specific for either β-NADH or β-NADPH are known. The pig heart enzyme of Straub seems to have native diaphorase (β-NADH specific) as well as lipoic and lipoamide dehydrogenase activities.

Unit Definition

One unit of diaphorase will oxidize 1.0 micromole of beta-NADH per minute at pH 7.5 at 25 degrees C, with the corresponding reduction of the appropriate electron acceptor.

Physical form

Supplied as a lyophilized powder containing trehalose.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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    Certificates of Analysis (COA)

    Lot/Batch Number
    SLBB5497V
    SLBB5497

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    Analysis of bisphenols in soft drinks by on-line solid phase extraction fast liquid chromatography-tandem mass spectrometry.
    Gallart-Ayala, et al.
    Analytica Chimica Acta, 683(2), 227-233 (2011)

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