D4668

Dextranase from Penicillium sp.

lyophilized powder, 100-250 units/mg protein

• Synonym(s): 1,6-α-D-Glucan 6-glucanohydrolase
• CAS Number: 9025-70-1
• EC Number: 3.2.1.11 (BRENDA, IUBMB)
• EC Number: 232-803-9
• MDL number: MFCD00130940
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• form: lyophilized powder
• Quality Level: 200
• specific activity: 100-250 units/mg protein
• composition: Protein, ~25% Lowry
• storage temp.: 2-8°C

Description

Application

Dextranase from Penicillium sp. can be used to modify alternan. Dextranase from Penicillium sp. has also been used in a study that explored the possibility that the Dex protein might adopt a fold unfamiliar to any known structure.

Biochem/physiol Actions

The product is an endodextranase that hydrolyzes α-(1,6)-glucosidic linkages in dextran. Dextrans are undesirable compounds synthesized from sucrose by microbial contaminants during sugar production. They increase viscosity of the flow and decrease industrial recovery. Dextranase has been used for hydrolyzing dextran at sugar mills in order to improve efficiency of sugar production.

Unit Definition

One unit will liberate 1.0 μmole of isomaltose (measured as maltose) per min at pH 6.0 at 37 °C, using dextran as substrate.

Physical form

Partially purified, lyophilized powder

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)