D7291
Deoxyribonuclease I RNase-free solution from bovine pancreas
Synonym(s):
DNase I, Deoxyribonuclease I, pancreatic DNase I, pancreatic deoxyribonuclease I
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
Recommended Products
biological source
bovine pancreas
Quality Level
grade
Molecular Biology
form
buffered aqueous glycerol solution
specific activity
10000 units/mg protein
mol wt
29.1 kDa
concentration
5—15 mg protein/mL
technique(s)
DNA extraction: suitable
suitability
suitable for molecular biology
UniProt accession no.
application(s)
cell analysis
foreign activity
RNase, none detected
storage temp.
−20°C
Gene Information
cow ... DNASE1(282217)
Related Categories
General description
Deoxyribonuclease I digests single- and double-stranded DNA to a mixture of mono and oligonucleotides carrying 5′phosphates and 3′OH termini. This catalytic activity is divalent ion-dependent. In the presence of Mg2+, DNase I hydrolyzes each strand of double-stranded DNA randomly and independently. In the presence of Mn2+, both strands can be cleaved.
Application
Deoxyribonuclease I RNase-free solution from bovine pancreas has been used to digest DNA from various samples.
Used in molecular biology applications for removing DNA during RNA purification, for preparing DNA for nick translation, and for DNA-protein interaction analysis by footprinting methods.
Components
DNase I is provided in a solution of 50% (v/v) glycerol, 20 mM sodium acetate (pH 6.5), 5 mM CaCl2, and 0.1 mM PMSF.
Unit Definition
One unit will cause a ΔA260 of 0.001 per min per mL reaction mixture using calf thymus DNA as substrate.
Other Notes
RNA treated with this DNase I should not be used to generate a cDNA library or used for RT-PCR. For these sensitive applications, it is recommended to use DNase I, Amplification Grade , Catalog Number AMP-D1.
inhibitor
Product No.
Description
Pricing
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
S Homburg et al.
The Journal of cell biology, 150(2), 293-307 (2000-07-26)
We present the first evidence for a fast activation of the nuclear protein poly(ADP-ribose) polymerase (PARP) by signals evoked in the cell membrane, constituting a novel mode of signaling to the cell nucleus. PARP, an abundant, highly conserved, chromatin-bound protein
Expression of anti-Mullerian hormone and its type II receptor in germ cells of maturing rat testis
Ohyama K, et al.
Endocrine Journal, EJ15-0370 (2015)
A Deoxyribonuclease from Chlamydomonas reinhardii:Purification and Properties
TAIT GCL and HARRIS WJ
European Journal of Biochemistry, 75(2), 357-364 (1977)
Stage-Specific Expression Profiles of Anti-Mullerian Hormone and its Type II Receptor in Germ Cells during Spermatogenic Cycle of Rats
Ohyama K, et al.
Journal of Steroids & Hormonal Science, 8(187), 2-2 (2017)
Site-specific protein-DNA photocrosslinking. Analysis of bacterial transcription initiation complexes.
N Naryshkin et al.
Methods in molecular biology (Clifton, N.J.), 148, 337-361 (2001-05-19)
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service