D9909

Dextran Sucrase from Leuconostoc mesenteroides

lyophilized powder, ≥100 units/mg protein

• CAS Number: 9032-14-8
• EC Number: 2.4.1.5 (BRENDA, IUBMB)
• MDL number: MFCD00130936
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: bacterial (Leuconostoc mesenteroides)
• Quality Level: 200
• form: lyophilized powder
• specific activity: ≥100 units/mg protein
• composition: Protein, ~15% Lowry
• solubility: H₂O: soluble 0.9-1.1 mg/mL, clear to slightly hazy, colorless to light yellow
• storage temp.: −20°C

Description

General description

Dextran Sucrase from Leuconostoc mesenteroides belongs to glycoside hydrolase family 70 (GH70). It functions through a retaining mechanism and uses two catalytic acidic residues. Dextran sucrase has a dextran binding site in the C-terminal domain.[1]

Application

Dextran Sucrase from Leuconostoc mesenteroides has been used:

• in immobilization on shirasu porous membrane (SPG) for dextran production[2]
• in the enzymatic synthesis of dextran nanoparticles at various pH range[1]
• in the immobilization with hydroxyapatite for dextran production[3]

Dextran sucrase from Leuconostoc mesenteroides has been used in a study to investigate the functional and structural characterization of α-(1→2) branching sucrase derived from DSR-E glucansucrase. Dextran sucrase from Leuconostoc mesenteroides has also been used in a study to investigate the bioengineering of Leuconostoc mesenteroides glucansucrases.

The enzyme from Sigma has been used to prepare immobilized sphere for the production of dextran from sucrose.[4]

Biochem/physiol Actions

Dextransucrases are glucansucrases that are able to produce dextran, a glucose polymer linked mainly through α1-6 bonds. However, α1-3, α1-6, α1-4 and α1-2 bonds are also found, in both the main chain and the branching linkages.[5] The peptide has approximately 1600 amino acids. The aspartic acid in position 551 is essential for catalytic activity, while glutamic acid 589 and aspartic acid 662 complement the catalytic triad.[6] The activity of dextransucrase is decreased by EDTA, and is restored by the addition of calcium ions. Zinc, cadmium, lead, mercury and copper ions are inhibitory to various degrees.[7]

Quality

Chromatographically purified

Unit Definition

One unit will liberate 1.0 μmole of fructose per min at 37 °C, pH 5.4.

Physical form

Lyophilized powder containing dextran, MES buffer salts and CaCl₂

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)