E3763

Resorufin ethyl ether

≥98% (TLC), powder

• Synonym(s): 7-Ethoxy-3H-phenoxazin-3-one, Ethoxyresorufin, O⁷-Ethylresorufin
• Empirical Formula (Hill Notation): C₁₄H₁₁NO₃
• CAS Number: 5725-91-7
• Molecular Weight: 241.24
• Beilstein/REAXYS Number: 225973
• MDL number: MFCD00037661
• UNSPSC Code: 12161501
• PubChem Substance ID: 24894527
• NACRES: NA.47

Properties

• Quality Level: 200
• assay: ≥98% (TLC)
• form: powder
• storage condition: (Tightly closed. Dry)
• technique(s): activity assay: suitable
• color: orange to red
• mp: 223-225 °C (lit.)
• solubility: chloroform: 9.80-10.20 mg/mL, clear, orange
• suitability: suitable for fluorescence
• storage temp.: −20°C
• SMILES string: CCOc1ccc2N=C3C=CC(=O)C=C3Oc2c1.Fc4c(F)c(F)c(OC(=O)CNC(=O)OCC5c6ccccc6-c7ccccc57)c(F)c4F
• InChI: 1S/C23H14F5NO4.C14H11NO3/c24-17-18(25)20(27)22(21(28)19(17)26)33-16(30)9-29-23(31)32-10-15-13-7-3-1-5-11(13)12-6-2-4-8-14(12)15;1-2-17-10-4-6-12-14(8-10)18-13-7-9(16)3-5-11(13)15-12/h1-8,15H,9-10H2,(H,29,31);3-8H,2H2,1H3
• InChI key: ZOSYTBPPLWBBKM-UHFFFAOYSA-N

Description

General description

Resorufin ethers are used as markers or substrates for different cytochrome P (CYP) isoforms. Resorufin ethyl ether is the preferred resorufin ether to be metabolized by microsomes of 3-methylcholanthrene treated animals.

Resorufin is the reduced product of resazurin, also termed as Alamar Blu. It exhibits fluorescence at longer wavelengths and is flexible for modification in its hydroxyl group.

Research area: Cell Signaling

Application

Resorufin ethyl ether is suitable for the study of dealkylating activity of cytochrome P450 isozymes 1A1 and 1A2, where arylalkyl and alkyl isothiocyanates, and their glutathione, cysteine, and A′-acetylcysteine conjugates are used as inhibitors. It is suitable to study the ethoxyresorufin-O-deethylase (EROD) activity of cytochrome P450 activity (CYP1A1).

Substrates

Fluorimetric substrate for cytochrome P450 linked enzyme, CXPIA1/2

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)