F4799

3xFLAG^™ Peptide

≥90% (HPLC/MS), lyophilized powder

• Synonym(s): Met-Asp-Tyr-Lys-Asp-His-Asp-Gly-Asp-Tyr-Lys-Asp-His-Asp-Ile-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, ddddk peptide, dykddddk peptide
• Empirical Formula (Hill Notation): C₁₂₀H₁₆₉N₃₁O₄₉S
• Molecular Weight: 2861.87
• MDL number: MFCD01863911
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• Product Name: 3X FLAG^® Peptide, lyophilized powder
• assay: ≥90% (HPLC/MS)
• Quality Level: 200
• form: lyophilized powder
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Recommended working concentration is 100 μg/ml for elute 3X FLAG fusion proteins from the ANTI-FLAG^® M2 affinity gel.

The 3X FLAG Peptide is a synthetic peptide of 23 amino acid residue. The Asp-Tyr-Lys-Xaa-Xaa-Asp motif is repeated three times in the peptide. Eight amino acids at the C-terminus make up the classic FLAG sequence (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys).

Application

For use in competitive elution of 3X FLAG^® fusion proteins from the ANTI-FLAG^® M2 monoclonal antibody (F1804) in solution or bound to agarose on the ANTI-FLAG^® M2 agarose affinity gel (A2220). This is achieved by Affinity Chromatography. For the gentle elution of 3X FLAG fusion proteins from the ANTI-FLAG^® M2 affinity gels. 1X FLAG Peptide (F3290) will not elute 3X FLAG fusion proteins from ANTI-FLAG^® affinity gels.


Learn more product details in our FLAG^® application portal.

Preparation Note

To prepare a stock solution, dissolve in TBS (50 mMTris-HCl, pH 7.4, with 150 mM NaCl) at a concentration of 5 mg/ml.

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)