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GE17-5054-01

HiTrap® SP Fast Flow

Cytiva 17-5054-01, pack of 5 × 1 mL

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About This Item

UNSPSC Code:
23151816
NACRES:
NA.56

Pricing and availability is not currently available.

ligand

sulphopropyl

description

Ion Exchanger Type (value)

packaging

pack of 5 × 1 mL

manufacturer/tradename

Cytiva 17-5054-01

parameter

flow rate
42 psi

bed size

7 mm × 25 mm

bed volume

1 mL

column I.D.

7 mm

matrix

6% cross-linked agarose

particle size

45-165 μm

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This Item
GE17-5053-01GE17-5157-01GE17-5156-01
matrix

6% cross-linked agarose

matrix

6% cross-linked agarose

matrix

6% cross-linked agarose

matrix

6% cross-linked agarose

parameter

flow rate, 42 psi

parameter

<4 mL/min flow rate, 42 psi

parameter

flow rate, 42 psi

parameter

42 psi

particle size

45-165 μm

particle size

45-165 μm

particle size

45-165 μm

particle size

45-165 μm

packaging

pack of 5 × 1 mL

packaging

pack of 5 × 1 mL

packaging

pack of 5 × 5 mL

packaging

pack of 5 × 5 mL

manufacturer/tradename

Cytiva 17-5054-01

manufacturer/tradename

Cytiva 17-5053-01

manufacturer/tradename

Cytiva 17-5157-01

manufacturer/tradename

Cytiva 17-5156-01

ligand

sulphopropyl

ligand

quaternary amine

ligand

sulphopropyl

ligand

quaternary amine

General description

HiTrap® SP FF is prepacked with SP Sepharose Fast Flow, and is a strong cation exchanger for small-scale protein purifications as well as screening of binding and elutions conditions.

Application

Q, SP, DEAE, and CM Sepharose Fast FLow are based on a robust, 6% highly cross-linked beaded Agarose matrix with good flow properties and high loading capacities. ANX Sepharose 4 Fast FLow (high sub) is based on 4% highly cross-linked beaded Agarose. This results in a medium with higher porosity, which is particularly useful for the purification of high molecular mass Proteins.

Features and Benefits

  • Convenient and affordable for fast, easy ion exchange separations either alone or connected in series.
  • The industry standard for ion exchange chromatography.
  • High fLow rates and good scale-up potential.
  • Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient.
  • Predictable scale-up

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (0.2 M sodium acetate in 20% ethanol)
Store at 4 to 30 °C (20% Ethanol + 0.2 M Sodium Acetate)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

pictograms

Flame

signalword

Warning

hcodes

Storage Class

3 - Flammable liquids


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    Articles

    This page shows how to perform a purification of His-tagged membrane proteins.

    This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

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    Protocols

    This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

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