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GW21169

Sigma-Aldrich

Anti-APAF1 antibody produced in chicken

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-Apoptotic protease-activating factor 1

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About This Item

MDL number:
MFCD02095846
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

chicken

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

human, mouse

manufacturer/tradename

Genway 15-288-21169

technique(s)

western blot: suitable

NCBI accession no.

NP_863658.1

UniProt accession no.

O14727

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... APAF1(317)

Immunogen

Immunogen Sequence: GI # 32483361, sequence 307-388
Recombinant apoptotic protease activating factor isoform d

Application

Anti-APAF1 antibody produced in chicken is suitable for western blotting analysis at a dilution of 1:500, for tissue or cell staining at a dilution of 1:200.

Biochem/physiol Actions

Apoptotic protease activating factor 1 is a human homolog of C. elegans CED-4 gene and is a key regulator gene of apoptosis. It acts as a central component of the intrinsic pathway of apoptosis. Loss of APAF-1 expression is involved with chemorefractory malignant melanoma and neuronal cell differentiation. Over expression of this gene is related to an undifferentiated state in the testicular germ cell tumor (TGCT) pathway and is involved in carcinogenesis of germ cell tumors. APAF-1, when bound to cytochrome c, may trigger the activation of caspase-3 leading to apoptosis.

Physical form

Solution in phosphate buffered saline containing 0.02% sodium azide.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number
039K2035
029K1746

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Reza Behjati et al.
Cancer science, 102(1), 267-274 (2010-10-28)
Apoptotic protease activating factor-1 (APAF-1) is a key regulator gene of apoptosis, located downstream from p53. Loss of APAF-1 expression is associated with chemorefractory malignant melanoma and neuronal cell differentiation. In order to make clear the function of APAF-1 in
H Zou et al.
Cell, 90(3), 405-413 (1997-08-08)
We report here the purification and cDNA cloning of Apaf-1, a novel 130 kd protein from HeLa cell cytosol that participates in the cytochrome c-dependent activation of caspase-3. The NH2-terminal 85 amino acids of Apaf-1 show 21% identity and 53%
Yelin Chen et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 34(46), 15327-15339 (2014-11-14)
Neuronal gene expression is modulated by activity via calcium-permeable receptors such as NMDA receptors (NMDARs). While gene expression changes downstream of evoked NMDAR activity have been well studied, much less is known about gene expression changes that occur under conditions

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