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I2282

Sigma-Aldrich

Anti-Insulin-Like Growth Factor-II antibody produced in goat

affinity isolated antibody, lyophilized powder

Synonym(s):

Anti-IGF-II

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About This Item

MDL number:
MFCD00162567
UNSPSC Code:
51111800
NACRES:
NA.41
Pricing and availability is not currently available.

biological source

goat

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized powder

species reactivity

mouse

technique(s)

indirect ELISA: 0.5-1.0 μg/mL
neutralization: suitable
western blot: 0.1-0.2 μg/mL

UniProt accession no.

P09535

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grade

certified reference material, pharmaceutical secondary standard

grade

certified reference material, pharmaceutical secondary standard

grade

certified reference material, pharmaceutical secondary standard

grade

certified reference material, pharmaceutical secondary standard

application(s)

pharmaceutical

application(s)

pharmaceutical

application(s)

pharmaceutical

application(s)

pharmaceutical

agency

traceable to USP 1457888

agency

traceable to USP 1367606

agency

traceable to USP 1133966

agency

traceable to USP 1457877

CofA

current certificate can be downloaded

CofA

current certificate can be downloaded

CofA

current certificate can be downloaded

CofA

current certificate can be downloaded

packaging

pkg of 100 mg

packaging

pkg of 50 mg

packaging

pkg of 100 mg

packaging

pkg of 150 mg

storage temp.

-10 to -25°C

storage temp.

2-30°C

storage temp.

2-8°C

storage temp.

-10 to -25°C

General description

Insulin-like growth factor II (IGF II) also refers as multiplication stimulating activity or MSA is a protein that is structurally similar to proinsulin. It is a potent mitogen for many cultured cells like mouse 3T3 cells, normal rat kidney cells, human or chicken fibroblasts and MCF-7 human breast carcinoma cells. IGF II possess mitogenic growth factor property and hence facilitates growth-promoting activity in embryonic development. Anti-Insulin-Like Growth Factor-II antibody can be used for neutralization assays. Goat anti-Insulin-Like Growth Factor-II antibody reacts specifically with recombinant mouse IGF-II. The product has also shown 25% (approx.) cross-reactivity with recombinant human IGF-II.

Immunogen

purified recombinant mouse insulin-like growth factor II, expressed in E. coli.

Application

Anti-Insulin-Like Growth Factor-II antibody can be used in ELISA and western blotting.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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    Certificates of Analysis (COA)

    Lot/Batch Number
    047K0725
    086K1484
    085K1778
    092K1589

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    Qing Qiu et al.
    Endocrinology, 148(10), 4803-4811 (2007-07-14)
    The process of posttranslational modifications of IGF-II likely has important physiological consequences. In addition to mature IGF-II, glycosylated proIGF-II(156-amino acid peptide) and two glycosylated big IGF-II forms, IGF-II(1-104) and IGF-II(1-87), have been identified in the human circulation. Due to lack
    K P Karey et al.
    Cancer research, 48(14), 4083-4092 (1988-07-15)
    A completely serum-free assay method has been used to compare the mitogenic activities of polypeptide growth factors and estrogens with MCF-7 and T47D human breast cancer cells in culture. The lines were maintained in a viable, slowly dividing condition in

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