I3642

Monoclonal Anti-Interleukin-1β antibody produced in mouse

clone 8516.311, purified immunoglobulin, lyophilized powder

• Synonym(s): Anti-IL-1β
• MDL number: MFCD00162514
• UNSPSC Code: 51111800
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 8516.311, monoclonal
• form: lyophilized powder
• species reactivity: human
• technique(s): flow cytometry: suitable; immunohistochemistry: 8-25 μg/mL using human PBMC′s; neutralization: suitable; western blot: 1 μg/mL
• isotype: IgG1
• UniProt accession no.: P01584
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... IL1B(3553)

Description

Specificity

The antibody will neutralize the biological activity of recombinant human IL-1β. It will not neutralize the activity of recombinant human IL-α, recombinant mouse IL-1α, or recombinant mouse IL-1β. When immobilized on a microplate, this antibody will capture both recombinant as well as natural human IL-1β. In immunoblotting, the antibody shows ~25% cross-reactivity with recombinant rat IL-1β and <5% cross-reactivity with recombinant mouse IL-1β and recombinant porcine IL-1β. Additionally, in immunoblotting, the antibody exhibits no cross-reactivity with recombinant mouse IL-α, recombinant human IL-1ra, recombinant mouse IL-1ra, or recombinant rat IL-1α.

Immunogen

purifed, E. coli-derived, recombinant human IL-1β.

Application

May used in immunoblotting, immunohistochemistry, ELISA, and neutralization assays

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.

Preparation Note

Monoclonal Anti-Interleukin-1(IL-1) (mouse IgG1 isotype) is purified from a mouse hybridoma utilizing Protein A affinity chromatography.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 13 - Non Combustible Solids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable