L2898

Lysostaphin from Staphylococcus staphylolyticus

aseptically filled

• Synonym(s): Glycyl-glycine Endopeptidase
• CAS Number: 9011-93-2
• EC Number: 3.4.24.75 (BRENDA, IUBMB)
• MDL number: MFCD00131556
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• sterility: aseptically filled
• Quality Level: 200
• form: powder
• specific activity: >500 units/mg protein
• mol wt: 25 kDa
• composition: Protein, 40-70% biuret
• antibiotic activity spectrum: Gram-positive bacteria
• mode of action: cell wall synthesis | interferes
• storage temp.: −20°C

Description

General description

Chemical structure: peptide

Lysostaphin is mainly liberated as a proenzyme. It is highly stable at pH 4 and temperature 5°C.

Biochem/physiol Actions

Lysostaphin exhibit a lytic action against Staphylococcus aureus. It possesses several functions of three major enzymes, such as, glycylglycine endopeptidase, endo-β-N-acetyl glucosamidase and N-acteyl muramyl-L-alanine amidase. Lysostaphin can be used to treat antibiotic-resistant staphylococcal infections. Lysostaphin being an antistaphylococcal agent, can be used as a preservative in the food industry and in clinical labs for rapid screening.

Lysostaphin is a zinc endopeptidase with a molecular weight of approximately 25 kDa. Because lysostaphin cleaves the polyglycine cross-links in the peptidoglycan layer of the cell wall of Staphylococcus species it has been found useful for cell lysis and also as a potential anti-microbial therapeutic.
pH Optimum for activity: ~7.5

Unit Definition

One unit will reduce the turbidity (A₆₂₀) of a suspension of Staphylococcus aureus cells from 0.250 to 0.125 in 10 min at pH 7.5 at 37 °C in a 6.0 ml reaction mixture.

Preparation Note

Prepared from L 7386

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)