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L6522

Sigma-Aldrich

Monoclonal Anti-Human Lambda Light Chains (Bound and Free) antibody produced in mouse

clone HP-6054, ascites fluid

Synonym(s):

Monoclonal Anti-Human Lambda Light Chains (Bound and Free)

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

Pricing and availability is not currently available.

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

secondary antibodies

clone

HP-6054, monoclonal

contains

15 mM sodium azide

technique(s)

immunohistochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: 1:1,000 using using 2 μg/ml of human IgG myeloma containing lambda light chains as the coating protein
radioimmunoassay: suitable
western blot: suitable

isotype

IgG2a

shipped in

dry ice

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This Item
H7789H0164SAB4501366
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

IgG fraction of antiserum

antibody form

affinity isolated antibody

UniProt accession no.

P10412

UniProt accession no.

P10412

UniProt accession no.

P68431

UniProt accession no.

P16401

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

General description

Monoclonal Anti-Human Lambda Light Chains (mouse IgG2a isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulins are composed of two heavy and two light polypeptide chains held together by noncovalent forces and usually by interchain disulfide bridges. The various types of human (and other mammalian) immunoglobulins contain one of the two existing light chain types, either κ or λ in which multiple structural differences are reflected in antigenic variety, mainly the N-terminal (variable) domain of the chains.

Specificity

The antibody is specific for human lambda (λ) light chains. This antibody is reactive with intact IgG (λ), IgA (λ), free lambda light chains and Bence-Jones lambda light chains. It is non-reactive with kappa light chains.

Immunogen

Purified human IgG myeloma proteins covalently coupled to polyaminostyrene (PAS) microbeads.

Application

Monoclonal Anti-Human Lambda Light Chains (Bound and Free) antibody produced in mouse has been used:
  • in immunohistochemistry
  • in enzyme-linked immunosorbent assay (ELISA) assay
  • in immunohistology
  • in radioimmunoassay (RIA)
  • in precipitation assays
  • in immunoblotting
  • as capture antibodies in quantitative ELISA[1]

Biochem/physiol Actions

Immunoglobulins (Ig) are produced by the B cells and play crucial role in host humoral immune response. A typical immunoglobulin consists of two heavy chains and two light chains. The two light chains are either κ or the λ chain. The B cells express one type of light chain during their life time.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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