M0650

Medium 199

With Earle′s salts, without ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture, 10X

• Synonym(s): Cell growth medium, M199 Medium, TCM 199
• UNSPSC Code: 12352207
• NACRES: NA.75

Properties

• Product Name: Medium 199, 10 ×, With Earle′s salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
• Quality Level: 400
• sterility: sterile-filtered
• form: liquid
• concentration: 10 ×
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• components: NaHCO₃: no; phenol red: yes; sodium pyruvate: no; HEPES: no; Earle’s salts (5% CO₂): yes; L-glutamine: no
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

Application

Medium 199 has been used:

• to culture human mesothelial cells
• as a component of the holding medium for the collection of embryos from pluriparous Boer goat
• in the preparation of spheroid-embedded collagen matrices

Reconstitution

Supplement with 0.1 g/L L-glutamine, 2.2 g/L sodium bicarbonate at 1×.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable