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MAK122

Sigma-Aldrich

Phospholipid Assay Kit

sufficient for 100 colorimetric or fluorometric tests

Synonym(s):

Phospholipid Quantification Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84
Pricing and availability is not currently available.

usage

sufficient for 100 colorimetric or fluorometric tests

application(s)

cosmetics
food and beverages
pharmaceutical

detection method

colorimetric
fluorometric

shipped in

dry ice

storage temp.

−20°C

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Raf-1 RBD Protein, GST, 300 µg GST fusion-protein, corresponding to the human Ras Binding Domain (RBD, residues 1-149) of Raf-1, expressed in E. coli. with purity 50% at full length molecular weight 42 kDa. For use in Affinity Binding Assays

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p38γ/SAPK3 Protein, active, 10 µg Active, recombinant GST fusion protein corresponding to full length human p38γ/SAPK3, for use in Kinase Assays.

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p38γ/SAPK3 Protein, active, 10 µg

biological source

human

biological source

human

biological source

human

biological source

human

technique(s)

affinity binding assay: suitable

technique(s)

activity assay: suitable (kinase)

technique(s)

activity assay: suitable

technique(s)

activity assay: suitable (kinase)

concentration

>50%

concentration

-

concentration

-

concentration

-

mol wt

Mw 42 kDa

mol wt

Mw 65 kDa

mol wt

-

mol wt

Mw 69 kDa

form

liquid

form

-

form

liquid

form

-

Gene Information

human ... RAF1(5894)

Gene Information

human ... RAF1(5894)

Gene Information

human ... MRAS(22808)

Gene Information

human ... PEBP1(5037)

General description

Phospholipids are a class of lipids which constitute a major component of cell membranes and play important roles in signal transduction. Most phospholipids contain one diglyceride, a phosphate group, and one choline.

In this assay, phospholipids (such as lecithin, lysolecithin and sphingomyelin) are enzymatically hydrolyzed to choline which is determined using choline oxidase and a H2O2-specific dye. This results in a colorimetric (570 nm)/ fluorometric (λex = 530/λem = 585 nm) product directly proportional to the phospholipid concentration in the sample. The range of linear detection is 3 - 200 μM for colorimetric assays and 0.6 - 20 μM for fluorometric assays.

Application

Phospholipid assay kit has been used in biochemical analysis[1] and phospholipid quantitation.[2][3][4][5]

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the detection of phospholipids in biological samples

Principle

In this assay, phospholipids (such as lecithin, lysolecithin and sphingomyelin) are enzymatically hydrolyzed to choline which is determined using choline oxidase and a H2O2-specific dye. This results in a colorimetric (570 nm)/ fluorometric (λex = 530/λem = 585 nm) product directly proportional to the phospholipid concentration in the sample. The range of linear detection is 3 - 200 μM for colorimetric assays and 0.6 - 20 μM for fluorometric assays.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334,H412

Hazard Classifications

Aquatic Chronic 3 - Resp. Sens. 1

Storage Class

10 - Combustible liquids

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    Certificates of Analysis (COA)

    Lot/Batch Number
    122CC01A27
    122CB11A03
    122CB07A29
    122CB03A17
    122CA10A12

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