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MSQC2

Sigma-Aldrich

MS QCAL Peptide Mix

lyophilized powder

Synonym(s):

MS Qual/Quant QC Mix, universal MS platform standard

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.24
Pricing and availability is not currently available.

form

lyophilized powder

analyte chemical class(es)

amino acids, peptides, proteins

technique(s)

HPLC: suitable

application(s)

food and beverages

format

multi-component solution

shipped in

ambient

storage temp.

2-8°C

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Show Differences

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This Item
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Anti-Fetuin A antibody, Mouse monoclonal clone FetA-M, purified from hybridoma cell culture

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Anti-Fetuin A antibody, Mouse monoclonal

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Anti-Fibronectin antibody, Mouse monoclonal

Anti-α Actinin antibody, Mouse monoclonal clone BM-75.2, purified from hybridoma cell culture

SAB4200813

Anti-α Actinin antibody, Mouse monoclonal

clone

FetA-M, monoclonal

clone

C3, monoclonal

clone

FN-15, monoclonal

clone

BM-75.2, monoclonal

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

species reactivity

human

species reactivity

human, canine, porcine

species reactivity

human, mouse

species reactivity

human

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

Gene Information

human ... AHSG(197)

Gene Information

human ... AFP(174)

Gene Information

human ... FN1(2335)

Gene Information

human ... ACTN1(87)

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

General description

QCAL was designed using the QconCAT technology and recombinantly expressed in E. coli.  The parent protein sequence of QCAL is as follows:

MGALRVFDEFKPLVEEPQNLIRVFDEFKPLVKPEEPQNLIRVFDEFKPLVKPEEKPQNLIRVFDEFKPLVKPEEKPQNKPLIRVFKPDEFKPLVKPEEKPQNKPLIRVFKPDEFKPLVKPEEKPQNKPLIKPRVFDEFQPLVEEPQNLIRGVNDNEEGFFSARGGVNDNEEGFFSARGGVNDNEEGFFSARGGVNDNEEGFFSARGGGVNDNEEGFFSARGGGVNDNEEGFFSARGGGVNDNEEGFFSARGGGVNDNEEGFFSARGGGVNDNEEGFFSARGVNDNEEGFFSAKGGGVNDNEEGFFSARAVMDDFAAFVEKAVMMDDFAAFVEKAVMMMDDFAAFVEKGLVKFVVPRALELFRIGDYAGIKEALDFFARYLGYLEQLLRVLYPNDNFFEGKLFTFHADICTLPDTEKALVALVLVPRGSLEVLFQGPIEGRTENLYFQGDDDDKALVALVHHHHHH

QCAL was subsequently digested with trypsin to give a core mixture of 22 peptides.

Application

MSQC2 is intended to act as a universal MS platform standard, by providing several elements for calibration and performance assessment, such as instrument resolution and linearity of signal detection.
This product is optimized to assess platform characteristics, including:
  • Repeatability/Reproducibility between runs
  • System stability (drift, chromatography, signal intensity, sensitivity, etc.)
  • Inter- and intra- platform and lab comparisons

Features and Benefits

General

Complexity
  • Defined mixture gives confidence in your instruments analysis

Components

Each vial contains 25 μg of lyophilized peptides that are derived from trypsin digestion of the protein concatamer QCAL.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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    Certificates of Analysis (COA)

    Lot/Batch Number
    SLCM2891
    SLCL9478
    SLCK8643
    SLCJ9770
    SLCJ5107

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    Julie M Pratt et al.
    Nature protocols, 1(2), 1029-1043 (2007-04-05)
    An important area of proteomics involves the need for quantification, whether relative or absolute. Many methods now exist for relative quantification, but to support biomarker proteomics and systems biology, absolute quantification rather than relative quantification is required. Absolute quantification usually
    Claire E Eyers et al.
    Journal of the American Society for Mass Spectrometry, 19(9), 1275-1280 (2008-07-05)
    If proteome datasets are to be collated, shared, and merged for higher level proteome analyses, there is a need for generally accepted strategies and reagents for optimization and standardization of instrument performance. At present, there is no single protein or

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