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N5288

Sigma-Aldrich

Anti-NQO1 (C-terminal) antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-DIA4, Anti-DT-diaphorase, Anti-Diaphorase 4, Anti-NAD (P)H:Menadione oxidoreductase 1, dioxin-inducible 1, Anti-NAD(P)H dehydrogenase, quinone 1, Anti-NMOR1

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
Pricing and availability is not currently available.

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~28 kDa

species reactivity

mouse, rat, human

concentration

~1 mg/mL

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feature

class A, graduation

feature

class A, graduation

feature

class A, graduation

feature

class A, graduation

manufacturer/tradename

Corning 7101-50

manufacturer/tradename

Corning 7101-5

manufacturer/tradename

Corning 7101-100

manufacturer/tradename

Corning 7101-1

material

glass

material

glass

material

glass

material

glass

capacity

50.0 mL

capacity

5.0 mL

capacity

100.0 mL

capacity

1.0 mL

packaging

pack of 2 ea

packaging

pack of 2 ea, case of 6 ea

packaging

pack of 2 ea, case of 6 ea

packaging

pack of 2 ea, case of 6 ea

volume measuring range

50.0 mL, accuracy: 0.05 mL

volume measuring range

5.0 mL, accuracy: 0.01 mL

volume measuring range

100.0 mL, accuracy: 0.08 mL

volume measuring range

1.0 mL, accuracy: 0.006 mL

General description

NADPH:quinone oxidoreductase (NQO1) is an FAD containing electron reductase that regulates the detoxification of quinones obtained from the phenolic metabolites of benzene. It is a homodimeric protein that can bind either of the reducing cofactors; NADPH or NADH . NQO1 regulates chemoprotection, neuroprotection, antitumor quinones and antioxidative defense mechanisms . NQO1 may be overexpressed in breast, colon, pulmonary and hepatic tumors.
NADPH:quinone oxidoreductase (NQO1, DTdiaphorase) is a 2-electron reductase. NQO1 is a FAD-containing protein that exists as a homodimer. It is ubiquitously expressed.

Specificity

Anti-NQO1 (C-terminal) antibody is specific for rat, mouse, and human NQO1. The immunizing peptide specifically inhibits the detection of NQO1 band by immunoblotting.

Immunogen

Synthetic peptide sequence corresponding to amino acid residues 261-274 of human NQO1, with N-terminal added cysteine conjugated to KLH. The corresponding sequence differs by one amino acid in mouse and rat.
synthetic peptide corresponding to amino acid resudues 261-274 of human NQO1 with N-terminal added cysteine conjugated to KLH. The corresponding sequence differs by one amino acid in mouse and rat.

Application

Anti-NQO1 (C-terminal) antibody may be used in immunological applications such as immunoprecipitation, immunofluorescence and immunoblotting (approximately 28 kDa).
Anti-NQO1 (C-terminal) antibody produced in rabbit has been used in:
  • western blotting
  • immunoprecipitation
  • immunofluorescence

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Biochem/physiol Actions

NADPH:quinone oxidoreductase (NQO1, DTdiaphorase) can bioactivate certain antitumor quinones. NQO1 has a role in antioxidant defense via the generation of antioxidant forms of ubiquinone and vitamin E. High levels of NQO1 gene expression has been observed in liver and lung tumors as compared to normal tissues of the same origin.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number
118M4793V
11M4793V
21180821
045M4782V
053M4753V

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A mutually inhibitory feedback loop between the 20S proteasome and its regulator, NQO1
Moscovitz O, et al.
Molecular Cell, 47, 76-86 (2012)
NAD(P)H:quinone oxidoreductase 1: role as a superoxide scavenger.
Siegel, D., et al.
J. Mol. Pharmacol., 65(5), 1238-1247 (2004)
NAD(P)H:quinone oxidoreductase 1 (NQO1): chemoprotection, bioactivation, gene regulation and genetic polymorphisms
Ross D, et al.
Chemico-Biological Interactions, 77-97 (2000)
Annette M Krais et al.
Archives of toxicology, 90(4), 839-851 (2015-05-23)
The tumour suppressor p53 is one of the most important cancer genes. Previous findings have shown that p53 expression can influence DNA adduct formation of the environmental carcinogen benzo[a]pyrene (BaP) in human cells, indicating a role for p53 in the
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