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P0029

Sigma-Aldrich

Protease from Bacillus sp.

Synonym(s):

Protamex®

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About This Item

EC Number:
3.4.21.14
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
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biological source

Bacillus sp.

Quality Level

form

granular

enzyme activity

>1.5 AU-N/g

storage temp.

2-8°C

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General description

Protease from Bacillus sp. is extensively useful in pharmaceutical, leather, food and waste processing industry. It is widely used in manufacturing protein hydrolysates. Analysis of Bacillus protease stability and activity showed that the enzyme was active at all pH values from 5.0-11.0 with 100% activity at 8.0. Optimal pH for protein stability was determined to be at 7.0 while the optimal temperature for stability was at 60°C with 95% activity maintained.

Application

Protease from Bacillus sp. has been used:
  • to evaluate protease inhibitor activity
  • in high yields in batch fermentation using a response surface methodology
  • in a study to investigate a biosensor to detect a protease biomarker from Bacillus licheniformis

Analysis Note

minimum activity 1.5 AU-N/G solid

Legal Information

Protamex is a registered trademark of Novozymes Corp.

pictograms

Health hazardExclamation mark

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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