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P0194

Sigma-Aldrich

Protein Kinase Cζ isozyme human

≥75% (SDS-PAGE), recombinant, expressed in baculovirus infected insect cells, buffered aqueous solution

Synonym(s):

Ca2+-activated phospholipid-dependent serine-threonine kinase ζ isozyme human, PKCζ human

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About This Item

EC Number:
UNSPSC Code:
12352202
NACRES:
NA.32

Pricing and availability is not currently available.

recombinant

expressed in baculovirus infected insect cells

Quality Level

assay

≥75% (SDS-PAGE)

form

buffered aqueous solution

enzyme activity

>800 units/mg protein

mol wt

76-80 kDa by SDS-PAGE

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PRKCZ(5590)

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This Item
913847914851914908
Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

particle size

600 nm

particle size

400 nm

particle size

400 nm

particle size

200 nm

form

liquid

form

-

form

liquid

form

liquid

application(s)

battery manufacturing

application(s)

battery manufacturing

application(s)

battery manufacturing

application(s)

battery manufacturing

concentration

10 % (w/w) in ethanol

concentration

10 % (w/w) in ethanol

concentration

20 % (w/w) in ethanol

concentration

20 % (w/w) in ethanol

pore size

non-porous

pore size

non-porous

pore size

non-porous

pore size

non-porous

Biochem/physiol Actions

PKCζ is a downstream enzyme in insulin signaling pathways.
Protein Kinase C (PKC) is a serine/threonine kinase that is activated intracellularly by signal transduction pathways that produce DAG from phosphatidylinositol diphosphate (PIP2) and phosphatidylcholine (PC) through the action of various activated phospholipases. Phorbol esters also stimulate PKC. At least 11 PKC isozymes have been identified that differ in primary structure, tissue distribution, subcellular localization, response to extracellular signals, and substrate specificity. The isozymes can be grouped into three subfamilies. Members of the first family require Ca2+ and phospholipid and include PKCα, βI, βII, and γ. Members of the second family are phospholipid-dependent but Ca2+-independent, and include PKCδ, ε, η, and θ. Members of the third family are not activated by either DAG or phorbol esters and include PKCξ, μ, and ι.
Phosphorylation appears to be an important mechanism of regulation of all PKCs. PKC plays a role in the regulation of cell transformation, growth, differentiation, ruffling, vesicle trafficking, apoptosis and gene expression.

Suitability

PKC ε can transfer 1100 nmole of phosphate to PKC ε substrate peptide per minute per mg of total protein at 30 °C.

Unit Definition

One unit will transfer 1 nanomole of phosphate to PKC epsilon substrate peptide per minute at pH 7.5 at 30 deg C.

Physical form

Solution in 20 mM HEPES, pH 7.5; 2 mM EDTA, 2 mM EGTA, 5 mM DTT, 250 mM NaCl, 0.05% Triton X-100, and 50% glycerol.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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