P1146

Phosphatase, Acid from potato

lyophilized powder, ≥3.0 units/mg solid

• Synonym(s): (Orthophosphoric-monoester phosphohydrolase acid optimum), Acid Phosphatase from potato
• CAS Number: 9001-77-8
• EC Number: 3.1.3.2 (BRENDA, IUBMB)
• EC Number: 232-630-9
• MDL number: MFCD00131847
• UNSPSC Code: 12352204
• eCl@ss: 42010102
• NACRES: NA.54

Properties

• form: lyophilized powder
• Quality Level: 300
• specific activity: ≥3.0 units/mg solid
• mol wt: 69 kDa
• shipped in: wet ice
• storage temp.: −20°C
• SMILES string: O(CC(O)C)CC#C
• InChI: 1S/C6H10O2/c1-3-4-8-5-6(2)7/h1,6-7H,4-5H2,2H3
• InChI key: GZCWLCBFPRFLKL-UHFFFAOYSA-N

Description

General description

Acid phosphatase from potato is a phosphomonoesterase, which can appear in multiple molecular forms of similar molecular mass but with different isoelectric points.

Application

Acid phosphatase from potato has been used in a study to assess the potential allergenicity of novel gene products. It has also been used in a study to remove eight phosphate groups from casein at a pH of 7.0.

The activity of potato acid phosphatase in various surfactant medias was examined. Bis(2- ethylhexyl)sodium sulfosuccinate, Brij 35, and SDS at 3mM inhibited phosphatase activity while the surfactant cetyltrimethylammonium bromide enhanced activity.

Biochem/physiol Actions

Acid phosphatases (APase) are a family of enzymes that non-specifically catalyze the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate at an optimum pH of 4 to 7.

Unit Definition

One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 4.8 at 37 °C.

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)