P1925

Protein A–Agarose macrobeads

aqueous suspension

• Synonym(s): Protein A resin
• MDL number: MFCD00165563
• UNSPSC Code: 41106500
• NACRES: NA.56

Properties

• form: aqueous suspension
• Quality Level: 200
• extent of labeling: ~6 mg per mL
• matrix activation: cyanogen bromide
• matrix spacer: 1 atom
• capacity: ~6 mg/mL binding capacity (human IgG)
• storage temp.: 2-8°C

Description

General description

Protein A is an immunoglobulin (Ig)-binding protein that might be suitable for low-pressure antibody isolation. It is used to purify large amounts of IgG and binds to the Fc part of the antibody at the CH₂–CH₃ interface. Protein A-agarose is used for affinity chromatography, antibody purification and characterization, and protein A, G and L resins.

Application

Protein A–Agarose macrobeads has been used in immunoprecipitation. It has been used to study the effects of protein A immunoadsorption in patients with chronic dilated cardiomyopathy as well as to study multiple sclerosis and gastric cancer.

Physical form

Suspension in 0.9% NaCl containing 15Mm Sodium Azide

Safety Information

• hcodes: H412
• pcodes: P273 - P501
• Hazard Classifications: Aquatic Chronic 3
• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)