P2860
Monoclonal Anti-PSF antibody produced in mouse
clone B92, purified from hybridoma cell culture
Synonym(s):
Mouse Anti-Polypyrimidine tract-binding protein-associated splicing factor
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About This Item
Recommended Products
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
B92, monoclonal
form
buffered aqueous solution
species reactivity
mouse, human
packaging
antibody small pack of 25 μL
concentration
~2 mg/mL
General description
Monoclonal Anti-PSF (mouse IgG1 isotype) is derived from the B92 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a C57BL/6J mouse immunized with a lysate of mouse bone marrow-derived stromal cell line. Polypyrimidine tract-binding protein-associated splicing factor (PSF) is an ubiquitous nuclear matrix protein. It is characterized with two consensus RNA-binding domains and an unusual amino terminus rich in proline and glutamine residues.
Immunogen
lysate of mouse bone marrow-derived stromal cell line.
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)
Immunoprecipitation (1 paper)
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)
Immunoprecipitation (1 paper)
Monoclonal Anti-PSF antibody produced in mouse has been used in:
- immunofluorescence
- immunohistochemistry
- immunoprecipitation
Biochem/physiol Actions
Polypyrimidine tract-binding protein-associated splicing factor (PSF) plays a pivotal role in mammalian spliceosome. PSF is also necessary for pre-mRNA splicing and transcription. The restricted expression of PSF suggests that it is involved in the control of neuronal-specific splicing events occurring at particular stages of neuronal differentiation and maturation. Monoclonal antibodies reacting specifically with PSF are useful tools for the molecular identification and characterization of the functional activity of PSF.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
Purified from culture supernatant of hybridoma cells grown in a bioreactor.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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Human PSF concentrates DNA and stimulates duplex capture in DMC1-mediated homologous pairing.
Yuichi Morozumi, Ryohei Ino
Nucleic Acids Research, 40(7), doi: 10-doi: 10 (2011)
Feng Chi et al.
PloS one, 7(4), e35862-e35862 (2012-04-27)
IbeA-induced NF-κB signaling through its primary receptor vimentin as well as its co-receptor PSF is required for meningitic E. coli K1 penetration and leukocyte transmigration across the blood-brain barrier (BBB), which are the hallmarks of bacterial meningitis. However, it is
Guillaume Cornelis et al.
RNA biology, 13(9), 826-836 (2016-06-18)
Long non-coding RNAs (lncRNAs) are widely expressed and play various roles in cell homeostasis. However, because of their low conservation at the sequence level, recapitulating lncRNA evolutionary history is often challenging. While performing an ultrastructural analysis of viral particles present
Cytoplasmic Relocalization of TAR DNA-Binding Protein 43 Is Not Sufficient to Reproduce Cellular Pathologies Associated with ALS In vitro
Wobst HJ, et al.
Frontiers in Molecular Neuroscience, 10(7) (2017)
Differential nuclear localization and nuclear matrix association of the splicing factors PSF and PTB
Meissner M, et al.
Journal of Cellular Biochemistry, 76(4), 559-566 (2000)
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