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P4056

Platelet-Derived Growth Factor-BB from rat

Name: Platelet-Derived Growth Factor-BB from rat
Brand: SIGMA

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Synonym(s):

PDGF-BB

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About This Item

MDL number:

MFCD01094933

UNSPSC Code:

12352202

NACRES:

NA.77

Pricing and availability is not currently available.

Product Name

Platelet-Derived Growth Factor-BB from rat, PDGF-BB, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

recombinant

expressed in E. coli

Quality Level

100

form

lyophilized powder

potency

1.62 ng/mL EC₅₀

quality

endotoxin tested

mol wt

~30 kDa

packaging

pkg of 5x50 μg
pkg of 50 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

Related Categories

Cell Culture & Analysis

Cell Culture Supplements & Reagents

Growth Factors and Cytokines

Molecular Biology & Functional Genomics

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This Item	EHU138521	EHU119591	EHU017861
EHU120731 MISSION^® esiRNA	EHU138521 MISSION^® esiRNA	EHU119591 MISSION^® esiRNA	EHU017861 MISSION^® esiRNA
Ensembl \| human accession no. ENSG00000161570	Ensembl \| human accession no. ENSG00000108688	Ensembl \| human accession no. ENSG00000108700	Ensembl \| human accession no. ENSG00000181374
product line MISSION^®	product line MISSION^®	product line MISSION^®	product line MISSION^®
Gene Information human ... CCL5(6352), CCL5(6352)	Gene Information human ... CCL7(6354), CCL7(6354)	Gene Information human ... CCL8(6355)	Gene Information human ... CCL13(6357), CCL13(6357)
esiRNA cDNA target sequence CGCTGTCATCCTCATTGCTACTGCCCTCTGCGCTCCTGCATCTGCCTCCCCATATTCCTCGGACACCACACCCTGCTGCTTTGCCTACATTGCCCGCCCACTGCCCCGTGCCCACATCAAGGAGTATTTCTACACCAGTGGCAAGTGCTCCAACCCAGCAGTCGTCTTTGTCACCCGAAAGAACCGCCAAGTGTGTGCCAACCCAGAGAAGAAATGGGTTCGGGAGTACATCAACTCTTTGGAGATGAGCTAGGATGGAGAGTCCTTGAACCTGAACTTACACAAATTTGCCTGTTTCTGCTTGCTCTTGTCCTAGCTTGGGAGGCTTCCCCTCACTATCCTACCCCACCCGCTCCTTGAAGGGCCCAGATTCTACCACACAGCAGCAGTTACAAAAACCTTCCCCAGGCTGGACGTGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGTGGGTGGATCACTTGAG	esiRNA cDNA target sequence ACCACCAGTAGCCACTGTCCCCGGGAAGCTGTAATCTTCAAGACCAAACTGGACAAGGAGATCTGTGCTGACCCCACACAGAAGTGGGTCCAGGACTTTATGAAGCACCTGGACAAGAAAACCCAAACTCCAAAGCTTTGAACATTCATGACTGAACTGAAAACAAGCCATGACTTGAGAAACAAATAATTTGTATACCCTGTCCTTTCTCAGAGTGGTTCTGAGATTATTTTAATCTAATTCTAAGGAATATGAGCTTTATGTAATAATGTGAATCATGGTTTTTCTTAGTAGATTTTAAAAGTTATTAATATTTTAATTTAATCTTCCATGGATTTTGGTGGGTTTTGAACATAAAGCCTTGGATGTATATGTCATCTCAGTGCTGTAAAAACTGTGGGATGCTCCTCCCTTCTCTACCTCATGGGGG	esiRNA cDNA target sequence TTGCTGAGCACAATCCCATACCCTTCAGCCTCTGCTCCACAGAGCCTAAGCAAAAGATAGAAACTCACAACTTCCTTGTTTTGTTATCTGGAAATTATCCCAGGATCTGGTGCTTACTCAGCATATTCAAGGAAGGTCTTACTTCATTCTTCCTTGATTGTGACCATGCCCAGGCTCTCTGCTCCCTATAAAAGGCAGGCAGAGCCACCGAGGAGCAGAGAGGTTGAGAACAACCCAGAAACCTTCACCTCTCATGCTGAAGCTCACACCCTTGCCCTCCAAGATGAAGGTTTCTGCAGCGCTTCTGTGCCTGCTGCTCATGGCAGCCACTTTCAGCCCTCAGGGACTTGCTCAGCCAGATTCAGTTTCCATTCCAATCACCTGCTGCTTTAACGTGATCAATAGGAAAATTCCTATCCAGAGGCTGGAGA	esiRNA cDNA target sequence GAGGAGCAGAGAGGCAAAGAAACATTGTGAAATCTCCAACTCTTAACCTTCAACATGAAAGTCTCTGCAGTGCTTCTGTGCCTGCTGCTCATGACAGCAGCTTTCAACCCCCAGGGACTTGCTCAGCCAGATGCACTCAACGTCCCATCTACTTGCTGCTTCACATTTAGCAGTAAGAAGATCTCCTTGCAGAGGCTGAAGAGCTATGTGATCACCACCAGCAGGTGTCCCCAGAAGGCTGTCATCTTCAGAACCAAACTGGGCAAGGAGATCTGTGCTGACCCAAAGGAGAAGTGGGTCCAGAATTATATGAAACACCTGGGCCGGAAAGCTCACACCCTGAAGACTTGAACTCTGCTACCCCTACTGAAATCAAGCTGGAGTACGTGAAATGACTTTTCCATTCTCCTCTGGCCTCCTCTTCT
form lyophilized powder	form lyophilized powder	form lyophilized powder	form lyophilized powder
NCBI accession no. NM_002985	NCBI accession no. NM_006273	NCBI accession no. NM_005623	NCBI accession no. NM_005408

General description

Platelet-derived growth factor (PDGF) is a 30 kDa cationic polypeptide and consists of two disulphide linked chains A and B.[1][2] PDGF is expressed as hetero and homodimers : PDGF-AB FDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD.[3]

Application

Platelet-Derived Growth Factor-BB from rat has been used as a growth supplement in mouse embryonic fibroblast culture, motoneuron-muscle co-culture and adult spinal cord neurons culture.[4][3]

Biochem/physiol Actions

Platelet-derived growth factor (PDGF) elicits multifunctional actions with a variety of cells. It is mitogenic to mesoderm-derived cells, such as dermal and tendon fibroblasts, vascular smooth muscle cells, glial cells, and chondrocytes.[5] PDGF is a potent chemoattractant and activator of neutrophils, monocytes, and fibroblasts.[6] PDGF activates smooth muscle relocalization and multiplication. PDGF mediated increased smooth muscle muscle proliferation might be associated with gene regulation.[7] It increases the synthesis of phospholipids, cholesterol esters, glycogen and prostaglandins, and modulates LDL (low density lipoprotein) receptor binding.

Other Notes

Dimer of the B chain of rat PDGF.

Physical form

Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% trifluoroacetic acid containing 2.5 mg bovine serum albumin.

Analysis Note

The biological activity is measured by its ability to stimulate ³H-thymidine incorporation in quiescent NR6R-3T3 fibroblasts.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Specification Sheet

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