P4404

Polyguanylic acid potassium salt

lyophilized powder

• Synonym(s): Poly(G) potassium salt
• CAS Number: 54684-83-2
• MDL number: MFCD00131976
• UNSPSC Code: 41106305
• NACRES: NA.51

Properties

• biological source: enzyme from (synthesis)
• Quality Level: 200
• assay: ≥95% (TLC)
• form: lyophilized powder
• quality: ≤5% free nucleotides
• solubility: water: 19.60-20.40 mg/mL, clear to slightly hazy, colorless to light yellow
• storage temp.: −20°C

Description

General description

Polyguanylic acid takes up a four-stranded helical structure. It is synthetically produced in Thermus thermophilus from guanosine diphosphate (GDP) and its synthesis is catalyzed by the enzyme polynucleotide phosphorylase.

Application

Polyguanylic acid (PolyG) is used to study the effects of ionizing radiation on formation and stability of G-quadruplex structures and as a target molecule for physicochemical studies of electronic excitation (vibrational spectra) of guanosine.

Polyguanylic acid potassium salt has been used:

• as a ligand for surface neuropilin-1 (NRP1) for internalization studies
• for intercalation studies with trisubstituted and disubstituted triazole-linked phenyl derivatives CL41, CL42 and CL2r50 using voltammetry method
• as a synthetic polynucleotide for agarose gel electrophoresis analysis

Biochem/physiol Actions

Polyguanylic acid acts as a ligand for scavenger receptor and reduces the surface expression neuropilin-1 in endothelial cells.

Preparation Note

Prepared from GDP using polynucleotide phosphorylase

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)