P4439

Phosphatase, Alkaline from porcine kidney

lyophilized powder, ≥100 DEA units/mg protein

• Synonym(s): Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
• CAS Number: 9001-78-9
• EC Number: 3.1.3.1 (BRENDA, IUBMB)
• EC Number: 232-631-4
• MDL number: MFCD00131849
• UNSPSC Code: 12352204
• eCl@ss: 42010105
• NACRES: NA.54

Properties

• form: lyophilized powder
• Quality Level: 200
• specific activity: ≥100 DEA units/mg protein
• composition: Protein, ≥50%
• storage temp.: −20°C

Description

Application

Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. Alkaline phosphatase is also used to prevent DNA self ligation and for radiolabeling.

Biochem/physiol Actions

Alkaline phosphatase is a hydrolase enzyme that dephosphorylates molecules such as nucleotides, proteins and alkaloids.

Packaging

Package size based on DEA units

Unit Definition

One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 9.8 at 37 °C.

Physical form

Lyophilized fromTris-HCl buffer, with magnesium chloride and zinc chloride, pH 8.0

Analysis Note

Protein determined by biuret.

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)