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RAB0332

Sigma-Aldrich

Human Lipocalin-2 / NGAL ELISA Kit

for serum, plasma, cell culture supernatant and urine

Synonym(s):

Lipocalin-2, NGAL, Neutrophil gelatinase-associated lipocalin 

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32
Pricing and availability is not currently available.

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type serum
sample type plasma
sample type cell culture supernatant(s)
sample type urine

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 4 pg/mL
standard curve range: 4.1-1000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... LCN2(3934)

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biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

Gene Information

human ... CHUK(1147)

Gene Information

human ... MDM2(4193)

Gene Information

human ... ULK1(8408)

Gene Information

human ... CLTCL1(8218)

clone

14A231, monoclonal

clone

2A10, monoclonal

clone

2H8, monoclonal

clone

X22, monoclonal

species reactivity

monkey, human, mouse

species reactivity

human

species reactivity

human

species reactivity

bovine, mouse, rat, human

antibody form

affinity isolated antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

General description

Lipocalin-2 (LCN2), a secreted glycoprotein, is an adipokine.[1][2] It is expressed in various cells like respiratory cells, intestinal epithelial cells, endothelial cells, renal tubular cells and cardiomyocytes.[3] The gene LCN2 is located on human chromosome 9q34.[4] The human lipocalin-2 ELISA (enzyme-linked immunosorbent assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human lipocalin-2 in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human Lipocalin-2

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Human Lipocalin-2/NGAL ELISA Kit has been used in the electrochemical immunoassay.[5] It has also been used to determine the neutrophil gelatinase-associated lipocalin (NGAL) levels in the urine.[6]

Biochem/physiol Actions

Lipocalin-2 (LCN2) has been implicated in a variety of processes including cell differentiation, tumorigenesis and apoptosis.[3] Polymorphisms in the expression of LCN2 are associated with many cancers such as breast, colorectal, pancreatic, ovarian, gastric, thyroid, ovarian, bladder and kidney cancers.[1]

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS
  • RABELADEELISA 5X Assay/Sample Diluent Buffer E (Item E2)
  • RABSTOP3ELISA Stop Solution (Item I)
  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
  • RABWASH420X Wash Buffer (Item B)

pictograms

Corrosion
GHS05

signalword

Warning

hcodes

H290

Hazard Classifications

Met. Corr. 1

Storage Class

8A - Combustible corrosive hazardous materials

  • Specification Sheet

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    0327K0327
    0308K0327
    0104J0327
    1128J0327
    1121J0327

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    Lipocalin 2, the TNF-like receptor TWEAKR and its ligand TWEAK act downstream of NFAT1 to regulate breast cancer cell invasion.
    Gaudineau B, et. al.
    Journal of Cell Science, 125, 4475-4475 (2012)
    Network based analyses of gene expression profile of LCN2 overexpression in esophageal squamous cell carcinoma
    Wu B, et al.
    Scientific reports, 5403-5403 (2014)
    Quantification of NGAL in Urine of Endurance Cycling Athletes
    Machado J C Q, et al.
    Journal of physical activity & health, 15(9), 679-682 (2018)
    Network based analyses of gene expression profile of LCN2 overexpression in esophageal squamous cell carcinoma
    Wu B, et al.
    Scientific Reports, 5403-5403 (2014)
    Thionine-coordinated BCN nanosheets for electrochemical enzyme immunoassay of lipocalin-2 on biofunctionalized carbon-fiber microelectrode
    Chen Z, et al.
    Sensors and Actuators B, Chemical (2018)
    View All Related Papers

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