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SigmaSpin Sequencing Reaction Clean-Up

post-reaction clean-up columns

Synonym(s):

sequencing clean up columns

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About This Item

UNSPSC Code:
41106300
NACRES:
NA.52
Pricing and availability is not currently available.
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Quality Level

packaging

pkg of 70 ea

working volume

100 μL

storage temp.

2-8°C

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1 of 4

This Item
D1947B4930823516
storage temp.

2-8°C

storage temp.

room temp

storage temp.

room temp

storage temp.

-

Quality Level

400

Quality Level

100

Quality Level

200

Quality Level

-

Application

Ideal for removing dye-terminator nucleotides and primers from sequencing reactions and radiolabeled nucleotides, primers, and fluorescent dyes from nucleic acid probe labeling reactions.
SigmaSpin Sequencing Reaction Clean-Up has been used in the synthesis of antisense RNA probe.[1]

Other Notes

SigmaSpin Post-Reaction Clean-Up Columns are ideal for lower throughput applications, such as clean-up of probe labeling reactions or small numbers of sequencing reactions. These columns can accept sample volumes up to 100 μL. Each column comes with a collection tube to collect the DNA during centrifugation.

SigmaSpin Post-Reaction Clean-Up columns provide a fast, simple, and highly efficient method for removing unincorporated dyes, excess salts, and other interfering reaction components. Each column is packed with a pre-hydrated size-exclusion resin, equilibrated with molecular biology grade water, and supplied in our unique column design. The column also includes a snap-cap bottom seal and an o-ring containing screw-cap to ensure that the resin remains hydrated. SigmaSpin has been tested in high-throughput genome centers and core facilities with ABI PRISM® 3700, 3100, 310 and 377. Each well can accept sample volumes up to 20 μL.
For additional information, please see www.sigma-aldrich.com/sequencing.

Legal Information

ABI PRISM is a registered trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
SigmaSpin is a trademark of Sigma-Aldrich Co. LLC

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Charge Transport in Conjugated Polymers with Pendent Stable Radical Groups.
Zhang Y, et al.
Chemistry of Materials (2018)
Paulina Powroznik et al.
Sensors (Basel, Switzerland), 20(2) (2020-01-19)
In the present work, we report the use of regioregular poly(3-hexyltiophene) polymer (RR-P3HT) as a potential light-activated material for sensing the chemical nerve agent simulant dimethyl methylphosphonate (DMMP). The electrical response of thick films of RR-P3HT, deposited by spray-coating method
Tomasz Jarosz et al.
Polymers, 11(2) (2019-04-10)
A type of graft copolymer based on polysiloxane and regioregular poly(3-hexylthiophene) (P3HT) has been synthesised and its properties have been studied alongside those of its parent conjugated polymer-regioregular P3HT. Electrochemical analysis has revealed more significant changes in conformation of the
Xiaoyu Li et al.
Nature communications, 8, 15909-15909 (2017-06-27)
Micelles formed by the self-assembly of block copolymers in selective solvents have attracted widespread attention and have uses in a wide variety of fields, whereas applications based on their electronic properties are virtually unexplored. Herein we describe studies of solution-processable
Kinga Kepska et al.
Chemicke zvesti, 72(1), 251-259 (2018-01-26)
The first comprehensive spectroelectrochemical account of the behaviour of regioregular (RR-P3HT) and statistical (ST-P3HT) poly(3-hexylthiophenes) in solution is presented, in contrast to the many reports dealing with P3HT films merely deposited from solution. The conducted experiments revealed that the two

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