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S7073

Sigma-Aldrich

SGK1 (60-end), active, GST tagged human

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32
Pricing and availability is not currently available.

recombinant

expressed in baculovirus infected Sf9 cells

Quality Level

200

product line

PRECISIO® Kinase

assay

≥70% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

99-135 nmol/min·mg

mol wt

~73 kDa

UniProt accession no.

O00141

shipped in

dry ice

storage temp.

−70°C

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This Item
218286435759W224928
(+)-Dihydrocarvone mixture of isomers

37275

(+)-Dihydrocarvone

(+)-Dihydrocarvone, mixture of isomers 98%

218286

(+)-Dihydrocarvone, mixture of isomers

(S)-(+)-Carvone 96%

435759

(S)-(+)-Carvone

D-Carvone ≥96%, FG

W224928

D-Carvone

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

400

density

0.928 g/mL at 20 °C (lit.)

density

0.929 g/mL at 25 °C (lit.)

density

0.96 g/mL at 25 °C (lit.)

density

0.96 g/mL at 25 °C (lit.)

refractive index

n20/D 1.471

refractive index

n20/D 1.471 (lit.)

refractive index

n20/D 1.497 (lit.)

refractive index

n20/D 1.497 (lit.)

form

liquid

form

liquid

form

liquid

form

-

optical activity

[α]20/D +20±2°, neat

optical activity

[α]22/D +17°, neat

optical activity

[α]23/D +55.0±7°, neat

optical activity

-

composition

n-(+)-dihydrocarvone, ~77%

composition

-

composition

-

composition

contains IFRA restricted Carvone

Biochem/physiol Actions

SGK1 is a member of the serum- and glucocorticoid-induced protein kinase that is activated in vitro by 3-phosphoinositide-dependent protein kinase-1 (PDK1) and in vivo in response to signals that activate phosphatidylinositol (PI) 3-kinase. SGK1 mRNA is expressed in all tissues and the level of SGK1 mRNA is increased by stimulation with serum or dexamethasone. SGK1 promotes cell survival by phosphorylating and inactivating FKHRL1. SGK and Akt display differences with respect to the efficacy with which they phosphorylate the three regulatory sites on FKHRL1.

Physical form

Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.2 5mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number
SLBW4715
SLBV5167
SLBS1841V

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