S7570

SGK2 Active human

≥75% (SDS-PAGE), aqueous glycerol solution, recombinant, expressed in E. coli

• Synonym(s): Serum/Glucocorticoid-Regulated Kinase 2
• MDL number: MFCD07371188
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• recombinant: expressed in E. coli
• Quality Level: 200
• assay: ≥75% (SDS-PAGE)
• form: aqueous glycerol solution
• specific activity: 100 units/mg protein (Bradford)
• mol wt: protein apparent mol wt ~66 kDa
• UniProt accession no.: Q9HBY8
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... SGK2(10110)

Description

Application

Kinase activity is measured as the molar amount of phosphate incorporated into the Akt/SGK substrate peptide (RPRAATF), per minute per mg protein at 30 °C using a final concentration of 50 μM [³²P] ATP.

Biochem/physiol Actions

SGK2 is a member of the serum- and glucocorticoid-induced kinases (SGK) which are serine-threonine kinases and belong to the "AGC" kinase subfamily, which includes protein kinases A, G, and C and has SGK2 enhances Na⁺/K⁺-ATPase activity in a variety of cells. In addition, SGK2 mimics the function of SGK1 and SGK3 and participates in the regulation of renal epithelial Na⁺ channel ENaC activity.

Unit Definition

One unit will incorporate one nanomole of phosphate into the AKT/SGK substrate peptide(RPRAATF) per minute at 30 °C and pH 7.2 using a final concentration of 50 μM [32P] ATP.

Physical form

Supplied at 50 μL in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM dithiothreitol (DTT), 0.1 mM EGTA, and 30% glycerol.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable