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S8633

Sigma-Aldrich

Sphingomyelinase from Staphylococcus aureus

buffered aqueous glycerol solution, 100-300 units/mg protein (Lowry)

Synonym(s):

Sphingomyelin choline phosphohydrolase, Sphingomyelin phosphodiesterase

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About This Item

CAS Number:
9031-54-3
EC Number:
3.1.4.12 (BRENDA, IUBMB)
MDL number:
MFCD00132362
UNSPSC Code:
12352204
NACRES:
NA.32
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form

buffered aqueous glycerol solution

Quality Level

200

specific activity

100-300 units/mg protein (Lowry)

storage temp.

2-8°C

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Sphingomyelinase from Staphylococcus aureus buffered aqueous glycerol solution, 100-300 units/mg protein (Lowry)

S8633

Sphingomyelinase from Staphylococcus aureus

Sphingomyelinase from Bacillus cereus buffered aqueous glycerol solution, ≥100 units/mg protein (Lowry)

S9396

Sphingomyelinase from Bacillus cereus

Sphingomyelinase from Bacillus cereus lyophilized powder, ≥100 units/mg protein

S7651

Sphingomyelinase from Bacillus cereus

Phosphatase, Acid from sweet potato ammonium sulfate suspension, ≥10.0 units/mg protein (modified Warburg-Christian)

P1435

Phosphatase, Acid from sweet potato

specific activity

100-300 units/mg protein (Lowry)

specific activity

≥100 units/mg protein (Lowry)

specific activity

≥100 units/mg protein

specific activity

≥10.0 units/mg protein (modified Warburg-Christian)

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

form

lyophilized powder

form

ammonium sulfate suspension

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

300

Application

Sphingomyelinase from Staphylococcus aureus has been used to:
  • induce neurotoxicity in rat cortical cultures to study the protective effects of minocycline[1]
  • determine the concentration of sphingomyelin from serum samples[2]
  • enhance sphingomyelinase activity to study PARK9-mediated exosome biogenesis[3]

Biochem/physiol Actions

Bacterial sphingomyelinase is active at neutral pH. When used in cell culture in vitro, it hydrolyzes the sphingomyelin on the outer leaflet of the plasma membrane and produces ceramide that is lipid-soluble. Sphingomyelinase is the key enzyme in the sphingomyelinase/ceramide pathway, which is implicated in the pathogenesis of several neurodegenerative disorders.[1]
Initiates the formation of sphingomyelin-based second messengers. Activates MAPK (mitogen-activated protein kinase) and SAPKs (stress-activated protein kinases); generates ceramide from sphingomyelin.

Unit Definition

One unit will hydrolyze 1.0 μmol of TNPAL-sphingomyelin per min at pH 7.4 at 37 °C.

Physical form

Solution in 50% glycerol containing 0.25 M phosphate buffer, pH 7.5

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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    Certificates of Analysis (COA)

    Lot/Batch Number
    11190450
    019M4067V
    048M4007V
    11160328
    086M4028V

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    Elin Rebecka Carlsson et al.
    Frontiers in endocrinology, 9, 172-172 (2018-06-21)
    Metabolic surgery is superior to lifestyle intervention in reducing weight and lowering glycemia and recently suggested as treatment for type 2 diabetes mellitus. Especially Roux-en-Y gastric bypass (RYGB) has been focus for much research, but still the mechanisms of action
    Measurement of sphingomyelin and ceramide cellular levels after sphingomyelinase-mediated sphingomyelin hydrolysis.
    P Santana et al.
    Methods in molecular biology (Clifton, N.J.), 105, 217-221 (1999-07-31)
    Anne M Brauweiler et al.
    The Journal of allergy and clinical immunology, 131(2), 421-427 (2012-12-19)
    The skin of patients with atopic dermatitis (AD) has defects in keratinocyte differentiation, particularly in expression of the epidermal barrier protein filaggrin. AD skin lesions are often exacerbated by Staphylococcus aureus-mediated secretion of the virulence factor α-toxin. It is unknown
    Lysosomal enzyme defects and Parkinson disease.
    Nutan Sharma
    Neurology, 80(17), 1544-1545 (2013-03-29)
    Benoît-Joseph Laventie et al.
    The Biochemical journal, 450(3), 559-571 (2013-01-04)
    PVL (Panton-Valentine leukocidin) and other Staphylococcus aureus β-stranded pore-forming toxins are important virulence factors involved in various pathologies that are often necrotizing. The present study characterized leukotoxin inhibition by selected SCns (p-sulfonato-calix[n]arenes): SC4, SC6 and SC8. These chemicals have no
    View All Related Papers

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