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S9514

Sigma-Aldrich

Superose® 12 Prep Grade

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About This Item

CAS Number:
97599-42-3
MDL number:
MFCD00166679
UNSPSC Code:
23151817
NACRES:
NA.56
Pricing and availability is not currently available.

form

suspension

Quality Level

100

particle size

20-40 μm (wet)

pore size

1,000-300,000 Da fractionation range (globular proteins)

storage temp.

2-8°C

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3192361.0723324-1440
Phenolphthalein ACS reagent

105945

Phenolphthalein

Phenolphthalein solution 0.5 wt. % in ethanol: water (1:1)

319236

Phenolphthalein solution

Phenolphthalein indicator ACS,Reag. Ph Eur

1.07233

Phenolphthalein

Phenolphthalein JIS special grade, ≥98.0%

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Phenolphthalein

technique(s)

titration: suitable

technique(s)

titration: suitable

technique(s)

-

technique(s)

-

grade

ACS reagent

grade

-

grade

ACS reagent

grade

JIS special grade

solubility

acetone: soluble

solubility

-

solubility

-

solubility

acetone: soluble

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

application(s)

-

form

powder

form

liquid

form

solid

form

solid

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

-

Application

Superose® 12 prep grade is used for protein chromatography, gel filtration chromatography and gel filtration media. Superose® 12 prep grade has been used to purify and characterize a haemolysin of Actinomyces pyogenes as well as a fibrinogenase from Vipera lebetina (desert adder) venom. Superose® 12 prep grade has also been used for the isolation and characterization of an extracellular protease of Actinomyces pyogenes.

Other Notes

Highly cross-linked beaded agarose

Physical form

Suspension in 20% ethanol
aqueous ethanol suspension

Legal Information

Superose is a registered trademark of Cytiva

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Hazard Classifications

Flam. Liq. 3

Storage Class

3 - Flammable liquids

wgk_germany

WGK 3

flash_point_f

100.4 - 109.4 °F

flash_point_c

38 - 43 °C

  • Specification Sheet

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    MKBR7330V
    107K0680
    056H0384
    030K1568
    028H0649

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    Sigma-Aldrich

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    Tianwei Tan et al.
    Biotechnology journal, 5(5), 505-510 (2010-05-05)
    Following its market introduction in 1982, the cross-linked 12% agarose gel media Superose 12 has become widely known as a tool for size exclusion chromatography of proteins and other biological macromolecules. In this review it is shown that, when appropriate
    A Van Tol et al.
    Journal of lipid research, 32(11), 1719-1728 (1991-11-01)
    The present study demonstrates very high levels of plasma lipids and high density lipoprotein (HDL) apolipoproteins (apoA-I and apoE) in female Nagase analbuminemic rats (NAR) fed a semi-synthetic diet in order to further increase the hyperlipidemia present in this strain.
    Jun Xu et al.
    Journal of chromatography. A, 1169(1-2), 235-238 (2007-09-28)
    (-)-Epigallocatechin gallate (EGCG) was purified in one step from a green tea polyphenol (GTP) crude extract by adsorption chromatography on a Superose 12 HR 10/30 column. The mobile phase used was a mixture of acetonitrile and water with an optimum
    S Amano et al.
    Calcified tissue international, 43(2), 88-91 (1988-08-01)
    We reported previously that an osteoblast-rich population of mouse calvarial cells treated with lipopolysaccharide produced on interleukin-1 (IL-1)-like cytokine that closely resembles IL-1. In the present study, we examined whether or not the IL-1-like cytokine stimulates bone resorption. As a
    A I Zijlstra et al.
    Gastroenterology, 110(6), 1926-1935 (1996-06-01)
    Many putative pronucleating proteins have been isolated from the biliary concanavalin A (con A)-binding fraction. The pronase resistance of the overall nucleating-promoting activity was almost never taken into consideration. The aim of this study was to identify the major pronase-resistant
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