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Key Documents

SAB2500503

Sigma-Aldrich

Anti-HAP1 antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-HAP2, Anti-HIP5, Anti-HLP, Anti-Huntingtin-associated protein 1, Anti-hHLP1

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About This Item

MDL number:
MFCD01633033
UNSPSC Code:
12352203
NACRES:
NA.41
Pricing and availability is not currently available.

biological source

goat

Quality Level

100

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

human

technique(s)

indirect ELISA: suitable
western blot: suitable

UniProt accession no.

P54257

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Anti-HAP1 antibody produced in goat affinity isolated antibody, buffered aqueous solution

SAB2500503

Anti-HAP1 antibody produced in goat

Anti-HAP1 antibody produced in rabbit purified immunoglobulin, buffered aqueous solution

SAB1411596

Anti-HAP1 antibody produced in rabbit

Anti-Nap1 (N-terminal) antibody produced in rabbit ~1 mg/mL, affinity isolated antibody, buffered aqueous solution

N3788

Anti-Nap1 (N-terminal) antibody produced in rabbit

Anti-HAP1 Antibody, clone 1B6 clone 1B6, from mouse

MABN2456

Anti-HAP1 Antibody, clone 1B6

Quality Level

100

Quality Level

100

Quality Level

200

Quality Level

-

biological source

goat

biological source

rabbit

biological source

rabbit

biological source

mouse

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

-

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

-

technique(s)

indirect ELISA: suitable, western blot: suitable

technique(s)

western blot: 1 μg/mL

technique(s)

western blot: 0.5-1 μg/mL using 3T3 cells lysate and HEK-293T cells expressing human Nap1

technique(s)

immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

General description

The gene HAP1 (huntingtin-associated protein 1) encodes a nuclear enzyme that is mainly expressed in neurons. It is the human homolog of exonuclease III protein from E. coli. The gene is mapped to human chromosome 17q21.2-q21.3.

Immunogen

Peptide with sequence C-RYDFRYSEDREQ from the internal region of the protein sequence according to NP_003940.2; NP_817084.1.

Biochem/physiol Actions

The gene HAP1 (huntingtin-associated protein 1) is a bifunctional enzyme that functions as a DNA repair enzyme as well as in redox activation of transcription factors. It binds to the Huntington′s disease (HD) protein huntingtin in a polyglutamine length-dependent manner. It associates with huntingtin via the cytoskeletal proteins dynactin and pericentriolar autoantigen protein 1 forming coiled-coils. It plays a role in vesicle trafficking by facilitating interactions among cytoskeletal, vesicular and motor proteins. It is an apurinic/apyrimidinic (AP) site-specific DNA repair endonuclease that removes the AP sites produced naturally or by cytotoxic drugs and radiation. It also has RNAse H activity and controls the redox state of certain proto-oncogene products, such as the transcription factor c-Jun. The HAP1 protein is a candidate for pathology associated with HD.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Supplied at 0.5 mg/mL in Tris saline with 0.02% sodium azide and 0.5% bovine serum albumin.

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pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H315,H319

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

  • Specification Sheet

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    Certificates of Analysis (COA)

    Lot/Batch Number
    7528P1

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    A role for the human DNA repair enzyme HAP1 in cellular protection against DNA damaging agents and hypoxic stress.
    Walker LJ
    Nucleic Acids Research, 22, 4884-4889 (1994)
    Assignment of the huntingtin (Hdh) gene, the huntingtin associated protein (Hap1) gene, and the huntingtin interacting protein (Hip1) gene to rat chromosomes 14, 10, and 12 by radiation hybrid mapping and fluorescent in situ hybridization.
    Zimdahl H
    Cytogenetics and Cell Genetics, 94, 101-104 (2001)
    Huntingtin-associated protein 1 (HAP1) interacts with the p150Glued subunit of dynactin.
    Engelender S
    Human Molecular Genetics, 6, 2205-2212 (1997)
    L J Walker et al.
    Molecular and cellular biology, 13(9), 5370-5376 (1993-09-01)
    The DNA binding activity of the c-jun proto-oncogene product is inhibited by oxidation of a specific cysteine residue (Cys-252) in the DNA binding domain. Jun protein inactivated by oxidation of this residue can be efficiently reactivated by a factor from

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