Skip to Content
MilliporeSigma
All Photos(1)

Key Documents

View All Documentation

SAB4200723

Sigma-Aldrich

Anti-Tryptophan Hydroxylase antibody, Mouse monoclonal

clone WH-3, purified from hybridoma cell culture

Synonym(s):

Anti-TPH, Anti-TpOH

Sign Into View Organizational & Contract Pricing

Select a Size

All Photos(1)

Select a Size

Change View

About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

WH-3, monoclonal

form

buffered aqueous solution

mol wt

antigen ~55 kDa

species reactivity

mouse, human, rabbit, sheep, rat

concentration

~1.0 mg/mL

technique(s)

immunoblotting: 2-4 μg/mL using rabbit pineal gland lysate
immunofluorescence: suitable
immunohistochemistry: suitable

General description

Anti-Tryptophan Hydroxylase antibody, Mouse monoclonal (mouse IgG3 isotype) is derived from the WH-3 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with recombinant rabbit Tryptophan Hydroxylase. Tryptophan Hydroxylase (TPH), also known as TpOH, TrpH or L-tryptophan tetrahydropteridine: oxygen oxidoreductase (EC 1.14.16.4) is a member of biopterin-dependent aromatic amino acid hydroxylases family, together with tyrosine hydroxylase and phenylalanine hydroxylase. This gene spanning 29kbp with 11 exons is mapped to human chromosome 11p15.3-p14. This 444 amino acid protein is expressed in the gut, spleen, thymus and also in the pineal gland and the pituitary.

Immunogen

recombinant rabbit Tryptophan Hydroxylase.

Application

Anti-Tryptophan Hydroxylase antibody, Mouse monoclonal has been used in:
  • immunoblotting
  • immunohistochemistry
  • immunofluorescence

Biochem/physiol Actions

Tryptophan Hydroxylase (TPH) is a rate-limiting tetramer enzyme in the synthetic pathway of neurotransmitter serotonin from L-tryptophan and is considered a key factor for the maintenance of normal serotonin transmission in the central nervous system. The tryptophan hydroxylase 1 gene (TPH1) catalyzes the synthesis of 5-hydroxytryptophan. Mutation in the TPH1 gene leads to schizophrenia, irritable bowel syndrome (IBS) in women, suicidal behavior and depressive disorders.The encoded protein plays a vital role in regulation of cardiovascular function.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
096M4785

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service