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SAB4200763

Sigma-Aldrich

Anti-Dystrophin Antibody

enhanced validation

mouse monoclonal, MANDRA1

Synonym(s):

Anti-DMD

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
Pricing and availability is not currently available.

Product Name

Anti-Dystrophin antibody, Mouse monoclonal, clone MANDRA1, purified from hybridoma cell culture

biological source

mouse

Quality Level

200

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

MANDRA1, monoclonal

form

buffered aqueous solution

mol wt

~427 kDa

species reactivity

zebrafish, rat, mouse, human

enhanced validation

independent
Learn more about Antibody Enhanced Validation

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1 of 4

This Item
S5192SML0481PZ0184
GSK2193874 ≥98% (HPLC)

SML0942

GSK2193874

SB 222200 ≥98% (HPLC), solid

S5192

SB 222200

Qc1 ≥98% (HPLC)

SML0481

Qc1

PF-04457845 ≥98% (HPLC)

PZ0184

PF-04457845

form

powder

form

solid

form

powder

form

powder

assay

≥98% (HPLC)

assay

≥98% (HPLC)

assay

≥98% (HPLC)

assay

≥98% (HPLC)

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

storage temp.

room temp

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

room temp

solubility

DMSO: >5 mg/mL, clear

solubility

DMSO: soluble ~28 mg/mL, H2O: insoluble

solubility

DMSO: 5 mg/mL (clear solution, warmed)

solubility

DMSO: 20 mg/mL

color

white to beige

color

light yellow

color

white to beige

color

white to beige

General description

Anti-Dystrophin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the MANDRA1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Dystrophin is a rod-shaped cytoskeletal protein located to the periphery (plasma membrane) of normal striated muscle fibers.
Dystrophin is encoded by the gene mapped to human chromosome Xp21.2-p21.1.

Specificity

Anti-Dystrophin antibody, Mouse monoclonal specifically recognizes an epitope located on the 128 amino acids at the end of the C-terminal domain of the human Dystrophin molecule (residues 3558-3684). The antibody reacts with Dystrophin from human, mouse, rat and zebrafish origin. The antibody is specific to dystrophin and does not react with -actinin or utrophin.

Application

Anti-Dystrophin antibody, Mouse monoclonal has been used in:
  • immunohistochemistry
  • immunoblotting
  • immunofluorescence
  • enzyme-linked immunosorbent assay (ELISA)

Biochem/physiol Actions

Dystrophin is absent, reduced or altered because of mutation in Duchenne and Becker muscular dystrophies (DMD/BMD) or in its homologue in mouse. Severe DMD is associated with a marked Dystrophin deficiency whereas patients with the milder form of BMD show less pronounced abnormalities of protein expression.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

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    Certificates of Analysis (COA)

    Lot/Batch Number
    0000103895
    057M4815V

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    Analysis of dystrophin gene deletions by multiplex PCR in Moroccan patients
    Sbiti A, et al.
    BioMed Research International, 2(3), 158-160 (2002)
    Duchenne muscular dystrophy and dystrophin: pathogenesis and opportunities for treatment
    Nowak KJ and Davies KE
    EMBO Reports, 5(9), 872-876 (2004)
    J P Hugnot et al.
    Proceedings of the National Academy of Sciences of the United States of America, 89(16), 7506-7510 (1992-08-15)
    A transcript generated by the distal part of the Duchenne Muscular Dystrophy (DMD) gene was initially detected in cells where the full size 14-kilobase (kb) messenger RNA is not found at a significant level. This transcript, approximately 4.5 kb long
    Meiling Zhang et al.
    PloS one, 10(7), e0132567-e0132567 (2015-07-18)
    Mitochondrial flash (mitoflash) is a highly-conserved, universal, and physiological mitochondrial activity in isolated mitochondria, intact cells, and live organisms. Here we investigated developmental and disease-related remodeling of mitoflash activity in zebrafish skeletal muscles. In transgenic zebrafish expressing the mitoflash reporter
    G E Morris et al.
    Journal of immunological methods, 161(1), 23-28 (1993-05-05)
    A novel approach to the quantitation of the muscular dystrophy protein, dystrophin, in muscle extracts is described. The two-site ELISA uses two monoclonal antibodies against dystrophin epitopes which lie close together in the rod domain of the dystrophin molecule in
    View All Related Papers

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