Skip to Content
MilliporeSigma
All Photos(4)

Key Documents

View All Documentation

SAB4200766

Sigma-Aldrich

Anti-Nitric Oxide Synthase, Inducible antibody, Mouse monoclonal

clone NOS-IN, purified from hybridoma cell culture

Synonym(s):

Anti-Hepatocyte NOS (HEP-NOS), Anti-Inducible NOS (iNOS), Anti-NOS type II, Anti-Peptidyl-cysteine S-nitrosylase NOS2

Sign Into View Organizational & Contract Pricing
All Photos(4)

About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
Pricing and availability is not currently available.

biological source

mouse

Quality Level

200

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

NOS-IN, monoclonal

form

buffered aqueous solution

mol wt

~130 kDa

species reactivity

human, mouse, rat, plant

concentration

~1.0 mg/mL

technique(s)

flow cytometry: suitable
immunoblotting: 2-4 μg/mL using mouse macrophage RAW 264.7 cell line activated with lipopolysaccharide (LPS) and interferon-γ
immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human pancreas sections

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
NWKITFLNWKITSQNWKITIE
Immobilon®-P NOW Bundle 0.45 um ( 0.45 micron filter ) pore size Hydrophobic immobilon-P PVDF Transfer Membrane for western blotting using Chemiluminescence, Chromogenic or Radioactive detection methods. Comes in an 8.5 cm x 10 m roll.

NWKITIP

Immobilon®-P NOW Bundle

Immobilon®-FL NOW Bundle 0.45 um ( 0.45 micron filter ) pore size Hydrophobic PVDF Transfer Membrane for western blotting using Fluorescent, Chemifluorescence, Chromogenic, or Chemiluminescence. Comes in a 8.5 cm x 10 m roll.

NWKITFL

Immobilon®-FL NOW Bundle

Immobilon®-PSQ NOW Bundle 0.2 um filter ( 0.2 micron ) pore size Hydrophobic PVDF Transfer Membrane for western blotting using Chemiluminescence, Chromogenic or Radioactive detection methods. Comes in an 8.5 cm x 10 m roll.

NWKITSQ

Immobilon®-PSQ NOW Bundle

Immobilon®-E NOW Bundle The first PVDF transfer Membrane for western blotting that wets out in transfer buffer or water. For chemiluminescence or chromogenic detection.

NWKITIE

Immobilon®-E NOW Bundle

material

PVDF membrane, white filter, plain filter

material

PVDF membrane, plain filter, white filter

material

PVDF membrane, plain filter, white filter

material

PVDF membrane, plain filter, white filter

pore size

0.45 μm

pore size

0.45 μm

pore size

0.20 μm pore size

pore size

0.45 μm pore size

detection method

chemiluminescent, radioactive, colorimetric

detection method

chemiluminescent, colorimetric, fluorometric, radioactive

detection method

chemiluminescent, colorimetric, radioactive

detection method

chemiluminescent, colorimetric, radioactive

capacity

160 μg/cm2 adsorption capacity(Insulin), 294 μg/cm2 adsorption capacity(Goat IgG), 215 μg/cm2 adsorption capacity(BSA)

capacity

155 μg/cm2 adsorption capacity(Insulin), 205 μg/cm2 adsorption capacity(BSA), 300 μg/cm2 adsorption capacity(Goat IgG)

capacity

262 μg/cm2 adsorption capacity(Insulin), 340 μg/cm2 adsorption capacity(BSA), 448 μg/cm2 adsorption capacity(Goat IgG)

capacity

-

technique(s)

western blot: suitable

technique(s)

western blot: suitable

technique(s)

dot blot: suitable, western blot: suitable

technique(s)

western blot: suitable

feature

hydrophobic

feature

hydrophobic

feature

hydrophobic

feature

easily wetted with water

General description

Anti-Nitric Oxide Synthase, Inducible antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the NOS-IN hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mouse. Inducible-type nitric oxide synthase (NOS) of mouse macrophage origin (iNOS) or macrophage NOS (mNOS) is a type II NOS (NOS2). It is a Ca2+-independent protein, expressed in activated macrophages and some glial cells after stimulation. NOS2 is also present in several other cell types including hepatocytes, chondrocytes, endothelial cells and fibroblasts. The iNOS gene is mapped to human chromosome 17q11.2. iNOS is a dimeric flavoprotein with a heme group. It comprises the C-terminal reductase domain and an N-terminal oxygenase domain.

Specificity

Anti-Nitric Oxide Synthase, Inducible antibody, Mouse monoclonal reacts specifically with inducible-type nitric oxide synthase (iNOS, also termed macNOS and mNOS). It does not react with NOS derived from brain (bNOS) or with endothelial-type NOS (eNOS). The antibody recognizes iNOS from human, mouse, rat and plant origin.

Immunogen

synthetic peptide corresponding to the C terminal region of nitric oxide synthase (NOS) of mouse macrophage origin (iNOS, also termed macNOS), conjugated to KLH

Application

Anti-Nitric Oxide Synthase, Inducible antibody, Mouse monoclonal may be used:
  • immunoblotting
  • immunofluorescence
  • immunohistochemistry
  • fluorescence-activated cell sorting (FACS)

Biochem/physiol Actions

Inducible nitric oxide synthase (iNOS) or NOS2 catalyzes the oxidation of L-arginine to L-citrulline and nitric oxide (NO) via an OH-L-arginine intermediate in the presence of reduced nicotinamide adenine dinucleotide phosphate (NADPH) and oxygen. Elevated levels of iNOS is observed in asthma. A high level of iNOS leads to an increase in NO production which may contribute to cell death and tissue damage. It is also implicated in the pathophysiology of septic shock. Single nucleotide polymorphism in the iNOS gene is indicative of high risk for B and T-cell non-Hodgkin lymphoma. iNOS also elicits macrophage bactericidal functionality.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    0000225100
    119M4781V
    089M4790V
    059M4859V
    077M4855V

    Don't see the Right Version?

    If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

    Search for a COA

    Already Own This Product?

    Find documentation for the products that you have recently purchased in the Document Library.

    Visit the Document Library

    Krystyna Boczoń et al.
    Folia histochemica et cytobiologica, 42(4), 209-213 (2005-02-12)
    The aim of this study was to demonstrate iNOS mRNA expression in muscular phase of experimental trichinellosis and to localize iNOS protein in T. spiralis-infected muscles using specific anti-iNOS monoclonal antibodies. The expression of iNOS mRNA in skeletal muscles from
    Christian Bogdan
    Trends in immunology, 36(3), 161-178 (2015-02-18)
    Thirty years after the discovery of its production by activated macrophages, our appreciation of the diverse roles of nitric oxide (NO) continues to grow. Recent findings have not only expanded our understanding of the mechanisms controlling the expression of NO
    Nitric oxide. No endothelial NO.
    S H Snyder
    Nature, 377(6546), 196-197 (1995-09-21)
    O Bagasra et al.
    Proceedings of the National Academy of Sciences of the United States of America, 92(26), 12041-12045 (1995-12-19)
    Nitric oxide (NO) has been implicated as a pathogenic mediator in a variety of central nervous system (CNS) disease states, including the animal model of multiple sclerosis (MS) and experimental allergic encephalomyelitis. We have examined post-mortem brain tissues collected from
    iNOS (NOS2) at a glance
    Lowenstein CJ and Padalko E
    Journal of Cell Science, 117(14), 2865-2867 (2004)
    View All Related Papers

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service