SAB4700423

Monoclonal Anti-CD326 antibody produced in mouse

clone VU-1D9, purified immunoglobulin, buffered aqueous solution

• Synonym(s): Anti-EPCAM
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: VU-1D9, monoclonal
• form: buffered aqueous solution
• species reactivity: human
• concentration: 1 mg/mL
• technique(s): western blot: suitable
• isotype: IgG1
• NCBI accession no.: NM_002354.2
• UniProt accession no.: P16422
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... EPCAM(4072)

Description

General description

Epithelial cell adhesion molecule (EpCAM) or cluster of differentiation 326 (CD326) is encoded by the 14 kb GA733-2 gene mapped to human chromosome 2p21. Human EpCAM expression is restricted to epithelia of colon, small intestine, lung, kidney, thymus, liver, pancreas, stomach. It is also expressed in several cancers, progenitor and stem cells. CD326 is a transmembrane glycoprotein characterized with an ectodomain, one transmembrane domain, and a 26 residual cytoplasmic domain.

The mouse monoclonal antibody VU-1D9 recognizes an epitope within EGF-like domain I of CD326 / EpCAM, a marker of epithelial lineages. This antibody strongly stains various normal epithelial cells and carcinomas.

Immunogen

Small cell lung carcinoma cell line H69

Application

Suggested working dilution for immunoblotting is 1-4 μg/mL of sample. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

Biochem/physiol Actions

Epithelial cell adhesion molecule (EpCAM, CD326) is a transmembrane glycoprotein, involved in several cellular functions, such as epithelial-specific intercellular cell–adhesion, cell signaling, migration, proliferation and differentiation. EpCAM is a dominant surface antigen mapped on human colon carcinoma. It has potential as a diagnostic marker for several carcinomas, including epithelial ovarian cancer (EOC). EpCAM is considered to be a potential target for cancer treatment. EpCAM with the help of components of wnt signal transduction pathway plays a vital role in stem cell signaling.

Features and Benefits

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Physical form

Solution in phosphate buffered saline, pH 7.4, with 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable