SAB4702020

Monoclonal Anti-CD79b PE produced in mouse

• Synonym(s): B29, BCR beta, IGB, Ig-beta
• UNSPSC Code: 12352203

Properties

• conjugate: phycoerythrin (R-PE) conjugate
• Quality Level: 100
• antibody product type: primary antibodies
• clone: CB3-1, monoclonal
• form: buffered aqueous solution
• species reactivity: human
• technique(s): flow cytometry: suitable
• isotype: IgG1κ
• UniProt accession no.: P40259
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... CD79B(974)

Description

General description

CD79b (Ig beta, B29) forms disulfide-linked heterodimer with CD79a (Ig alpha, MB1). They both are transmembrane proteins with extended cytoplasmic domains containing immunoreceptor tyrosine activation motives (ITAMs), and together with cell surface immunoglobulin they constitute B-cell antigen-specific receptor (BCR). CD79a and b are the first components of BCR that are expressed developmentally. They appear on pro-B cells in association with the endoplasmic reticulum chaperone calnexin. Subsequently, in pre-B cells, CD79 heterodimer is associated with lambda5-VpreB surrogate immunoglobulin and later with antigen-specific surface immunoglobulins. CD79a/b complex interacts with Src-family tyrosine kinase Lyn, which phosphorylates its cytoplasmic ITAM motives to form docking sites for downstream signaling.

Specificity

The mouse monoclonal antibody CB3-1 recognizes an extracellular epitope of CD79b (CD79 beta, Ig beta), an approximately 38 kDa component of B cell receptor (BCR) complex.

Immunogen

Fraction of Ig-associated molecules isolated from Ramos B cells

Physical form

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preparation Note

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Storage and Stability

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Other Notes

The antibody solution should be gently mixed before use.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable