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Key Documents

SAB4702021

Monoclonal Anti-Cyclin D1 PE produced in mouse

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Synonym(s):

Bcl-1, CCND1, CYC-D1, PRAD1, U21B31

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About This Item

UNSPSC Code:
12352203

conjugate

phycoerythrin (R-PE) conjugate

Quality Level

antibody product type

primary antibodies

clone

DCS-6, monoclonal

form

buffered aqueous solution

species reactivity

( Human), ( Mouse), rat, ( Non-human primates)

concentration

0.1 mg/ml

technique(s)

flow cytometry: suitable

isotype

IgG2a

UniProt accession no.

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... CCND1(595)

General description

Cyclin D1 (PRAD1, Bcl-1) is a cytoplasmic and nuclear protein, which is synthesized during G1 phase and assembles with either cyclin-dependent kinase 4 (CDK4) or CDK6 in response to growth factor stimulation. D-type cyclin-CDK complexes act to inactivate the growth-suppressive function of the Rb protein through its phosphorylation, and titrate CDK inhibitors such as p21Cip1 and p27Kip1. Whereas during G1 phase cyclin D1 accumulates in the nucleus, it translocates into the cytoplasm during S phase. Without growth factor-mediated stimulation, cyclin D1 is unstable, and undergoes ubiquitin-mediated degradation, which is triggered by its phosphorylation. Cyclin D1 destabilization participates in G1/S phase arrest.

Specificity

The mouse monoclonal antibody DCS-6 recognizes cyclin D1, an ubiquitously expressed 33 kDa intracellular protein that migrates as a 36 kDa band under reducing SDS-PAGE conditions.

Immunogen

recombinant human cyclin D1 (amino acids 1-295)

Physical form

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preparation Note

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Storage and Stability

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Other Notes

The antibody solution should be gently mixed before use.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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