SAB5702586

Anti-ULK3 Antibody, clone 5Z7Z6, Rabbit Monoclonal

• Synonym(s): unc-51 like kinase 3
• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• biological source: rabbit
• Quality Level: 100
• material: colorless
• clone: 5Z7Z6, monoclonal
• form: liquid
• species reactivity: rat, rat, human
• concentration: 0.70 mg/mL
• technique(s): immunofluorescence: 1:50 - 1:200; western blot: 1:500 - 1:2000
• color: colorless
• isotype: IgG
• immunogen sequence: KPRLLAALEVASAAMAKEEAAGGEQDALDLYQHSLGELLLLLAAEPPGRRRELLHTEVQNLMARAEYLKEQVKMRESRWEADTLDKEGLSESVRSSCTLQ
• UniProt accession no.: Q6PHR2
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... ULK3(25989)

Description

General description

Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3. Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy.

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 373-472 of human ULK3 (Q6PHR2).

PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.

Application

WB, IF/ICC

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1