SAE0156
Bone Alkaline Phosphatase from human bone osteosarcoma cells
lyophilized, ≥2000 u/mg protein
Synonym(s):
ALPL, BAP, Bone ALP, Ostase
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About This Item
UNSPSC Code:
12352204
NACRES:
NA.77
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This Item | 8.52043 | 8.52049 | 8.52074 |
|---|---|---|---|
| form powder | form powder | form powder | form powder |
| assay ≥90.0% (acidimetric), ≥98.0% (HPLC), ≥98% (TLC) | assay ≥96.0% (HPLC), ≥96.0% (acidimetric), ≥98% (TLC) | assay ≥95.0% (acidimetric), ≥98% (TLC), ≥99.0% (HPLC) | assay ≥94.0% (acidimetric), ≥97.0% (HPLC), ≥98% (TLC), ≥98% (perchloric acid titration) |
| functional group Fmoc | functional group Fmoc | functional group Fmoc | functional group Boc, amine |
| product line Novabiochem® | product line Novabiochem® | product line Novabiochem® | product line Novabiochem® |
| storage temp. 2-30°C | storage temp. 2-30°C | storage temp. 2-30°C | storage temp. 2-30°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
General description
Mammalian alkaline phosphatases are a group of membrane-bound glycoprotein isoenzymes that are attached to the cell membrane by a hydrophobic glycosyl-phosphatidylinositol (GPI) anchor.
The bone alkaline phosphatase isozyme is expressed from the gene for tissue nonspecific alkaline phosphatase which is also expressed in liver and kidney. These three isoforms form the majority of alkaline phosphatases circulating in serum. All three forms share the same amino acid sequence but have different carbohydrate and lipid modifications that provide unique properties according to the source tissue.
Bone alkaline phosphatase is synthesized by osteoblasts, involved in the calcification of bone matrix, but its precise role in bone formation process is still unknown. It is a highly specific marker of the bone-forming activity of osteoblasts. High activity of bone alkaline phosphatase is observed in serum in several bone diseases, such as Paget′s disease, osteoporosis, ricket disease, bone metastatic carcinoma, and others. Physiological bone growth can also contribute to increased levels of bone alkaline phosphatase in serum.
This product is purified from human osteosarcoma Saos-2 cells, an established cell line with high basal alkaline phosphatase activity. Before purification, alkaline phosphatase is released from the cell membrane by enzymatic cleavage of the GPI anchor.
The bone alkaline phosphatase isozyme is expressed from the gene for tissue nonspecific alkaline phosphatase which is also expressed in liver and kidney. These three isoforms form the majority of alkaline phosphatases circulating in serum. All three forms share the same amino acid sequence but have different carbohydrate and lipid modifications that provide unique properties according to the source tissue.
Bone alkaline phosphatase is synthesized by osteoblasts, involved in the calcification of bone matrix, but its precise role in bone formation process is still unknown. It is a highly specific marker of the bone-forming activity of osteoblasts. High activity of bone alkaline phosphatase is observed in serum in several bone diseases, such as Paget′s disease, osteoporosis, ricket disease, bone metastatic carcinoma, and others. Physiological bone growth can also contribute to increased levels of bone alkaline phosphatase in serum.
This product is purified from human osteosarcoma Saos-2 cells, an established cell line with high basal alkaline phosphatase activity. Before purification, alkaline phosphatase is released from the cell membrane by enzymatic cleavage of the GPI anchor.
Unit Definition
One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate to p-nitrophenol and inorganic phosphate per minute at pH 9.8 at 37 °C.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
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Timothy J Lansing et al.
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Cellular signalling, 27(5), 951-960 (2015-02-17)
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T Ikezoe et al.
Leukemia, 23(9), 1564-1576 (2009-05-08)
Polo-like kinase1 (PLK1) belongs to the family of serine/threonine kinases and plays an important role in centrosome maturation, bipolar spindle formation, and cytokinesis during mitosis. We found in this study that PLK1 was aberrantly highly expressed in a variety of
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Mitosis is choreographed by a number of protein kinases including polo-like kinases and Aurora kinases. As these kinases are frequently dysregulated in cancers, small-molecule inhibitors have been developed for targeted anticancer therapies. Given that PLK1 and Aurora kinases possess both
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