SML0430

PR-619

≥95% (HPLC)

• Synonym(s): 2,6-Diamino-3,5-dithiocyanopyridine, Thiocyanic acid C,C′-(2,6-diamino-3,5-pyridinediyl) ester; 2,6-Diaminopyridine-3,5-bis(thiocyanate)
• Empirical Formula (Hill Notation): C₇H₅N₅S₂
• CAS Number: 2645-32-1
• Molecular Weight: 223.28
• MDL number: MFCD00830384
• UNSPSC Code: 12352200
• PubChem Substance ID: 329825425
• NACRES: NA.77

Properties

• Quality Level: 100
• assay: ≥95% (HPLC)
• form: powder
• color: white to beige
• solubility: DMSO: 5 mg/mL (clear solution)
• storage temp.: −20°C
• SMILES string: Nc1nc(N)c(SC#N)cc1SC#N
• InChI: 1S/C7H5N5S2/c8-2-13-4-1-5(14-3-9)7(11)12-6(4)10/h1H,(H4,10,11,12)
• InChI key: ZXOBLNBVNROVLC-UHFFFAOYSA-N

Description

Application

PR-619 has been used:

• as a component of lysis buffer and also as a deubiquitinase inhibitor to treat proteins derived from SILAC-labeled Jurkat cells[1]
• as a pan-deubiquitinase inhibitor to study its effect on adenoassociated virus (AAV) transduction[2]
• in radioimmunoprecipitation assay buffer (RIPA) buffer used for ubiquitination assays[3]

Biochem/physiol Actions

PR-619 cell permeable, broad spectrum deubiquitylating enzymes (DBUs) inhibitor; general deubiquitylase (DUB) inhibitor.

PR-619 is a cell permeable broad spectrum deubiquitylating enzymes (DBUs) inhibitor. PR-619 induces the accumulation of polyubiquitylated proteins in cells without directly affecting proteasome activity.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3