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MilliporeSigma

SML0430

Sigma-Aldrich

PR-619

≥95% (HPLC)

Synonym(s):

2,6-Diamino-3,5-dithiocyanopyridine, Thiocyanic acid C,C′-(2,6-diamino-3,5-pyridinediyl) ester; 2,6-Diaminopyridine-3,5-bis(thiocyanate)

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About This Item

Empirical Formula (Hill Notation):
C7H5N5S2
CAS Number:
Molecular Weight:
223.28
MDL number:
UNSPSC Code:
12352200
PubChem Substance ID:
NACRES:
NA.77

Pricing and availability is not currently available.

Quality Level

assay

≥95% (HPLC)

form

powder

color

white to beige

solubility

DMSO: 5 mg/mL (clear solution)

storage temp.

−20°C

SMILES string

Nc1nc(N)c(SC#N)cc1SC#N

InChI

1S/C7H5N5S2/c8-2-13-4-1-5(14-3-9)7(11)12-6(4)10/h1H,(H4,10,11,12)

InChI key

ZXOBLNBVNROVLC-UHFFFAOYSA-N

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This Item
P09500005675377699
technique(s)

HPLC: suitable, gas chromatography (GC): suitable

technique(s)

-

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

technique(s)

-

application(s)

pharmaceutical (small molecule)

application(s)

pharmaceutical (small molecule)

application(s)

environmental

application(s)

-

format

neat

format

neat

format

neat

format

-

Quality Level

300

Quality Level

-

Quality Level

100

Quality Level

200

grade

certified reference material, pharmaceutical secondary standard

grade

pharmaceutical primary standard

grade

analytical standard

grade

-

storage temp.

2-30°C

storage temp.

2-8°C

storage temp.

-

storage temp.

-

Application

PR-619 has been used:
  • as a component of lysis buffer and also as a deubiquitinase inhibitor to treat proteins derived from SILAC-labeled Jurkat cells[1]
  • as a pan-deubiquitinase inhibitor to study its effect on adenoassociated virus (AAV) transduction[2]
  • in radioimmunoprecipitation assay buffer (RIPA) buffer used for ubiquitination assays[3]

Biochem/physiol Actions

PR-619 cell permeable, broad spectrum deubiquitylating enzymes (DBUs) inhibitor; general deubiquitylase (DUB) inhibitor.
PR-619 is a cell permeable broad spectrum deubiquitylating enzymes (DBUs) inhibitor. PR-619 induces the accumulation of polyubiquitylated proteins in cells without directly affecting proteasome activity.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3


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Maria Lauda Tomasi et al.
Oncotarget, 8(45), 78851-78869 (2017-11-08)
MicroRNA-34a (miR-34a) is down-regulated in colorectal cancers (CRC) and required for interleukin-6 (IL-6)-induced CRC metastasis. Mice lacking miR-34a developed more invasive cancer in a colitis-associated cancer model. In the same model, S-adenosylmethionine (SAMe) and methylthioadenosine (MTA) inhibited IL-6/STAT3 and lowered

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Utilize these Caco-2 cell based assay tools for screening small molecule drug compounds prior to clinical studies and submission to regulatory agencies.

Application note on Drug transport assays in a 96-well system using Millicell-96 System from Millipore.

We presents an article on The Role of Intestinal Efflux Transporters In Drug Absorption.

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