Skip to Content
MilliporeSigma
All Photos(5)

Key Documents

SRE0001

Sigma-Aldrich

Pepsin from porcine gastric mucosa

Suitable for manufacturing of diagnostic kits and reagents, lyophilized powder, ≥3200 units/mg protein

Synonym(s):

Pepsin A, Pepsin from hog stomach

Sign Into View Organizational & Contract Pricing


About This Item

CAS Number:
EC Number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

Pricing and availability is not currently available.

Quality Level

form

lyophilized powder

specific activity

≥3200 units/mg protein

mol wt

35 kDa

impurities

salt, essentially free

color

white to off-white

UniProt accession no.

application(s)

diagnostic assay manufacturing

shipped in

wet ice

storage temp.

−20°C

Looking for similar products? Visit Product Comparison Guide

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
909247959990925
suitability

enterobacteriaceae

suitability

selective and differential for Escherichia coli, selective and differential for coliforms

suitability

Enterococcus spp.

suitability

selective and differential for Klebsiella spp.

form

powder

form

powder

form

powder

form

powder

composition

agar, 15.0 g/L , chromogenic mixture, 4.0 g/L , sodium chloride, 5.0 g/L , buffer mix, 4.0 g/L , peptone mix, 12.0 g/L

composition

agar, 15.0 g/L , chromogen, 0.5 g/L , dipotassium phosphate, 3.3 g/L , lactose, 10.0 g/L , monopotassium phosphate, 1.0 g/L , proteose peptone, 5.0 g/L , sodium chloride, 7.5 g/L , sodium deoxycholate, 0.1 g/L , sodium lauryl sulfate, 0.2 g/mL , yeast extract, 3.0 g/L

composition

agar, 15.0 g/L , buffering agent, 1.25 g/L , chromogenic mixture, 0.45 g/L , peptone special, 25.0 g/L , salt mixture, 4.25 g/L , sodium chloride, 5.0 g/L

composition

agar, 15.0 g/L , bile salts mixture, 1.5 g/L , chromogenic mixture, 0.2 g/L , petone, special, 12.0 g/L , sodium chloride, 5.0 g/L , sodium lauryl sulfate, 0.1 g/L , yeast extract, 7.0 g/L

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

application(s)

agriculture
clinical testing
environmental
food and beverages
veterinary, microbiology

application(s)

agriculture
bioburden testing
environmental
food and beverages
water monitoring, microbiology

application(s)

clinical testing
environmental
food and beverages
veterinary, microbiology

application(s)

bioburden testing
clinical testing
environmental
food and beverages
water monitoring, microbiology

final pH

6.8±0.2 (25 °C)

final pH

7.3±0.2 (25 °C)

final pH

6.5±0.2 (25 °C)

final pH

7.1±0.2 (25 °C)

Application

Pepsin cleavage can be used to produce F(ab′)2 fragments of antibodies. pepsin at www.sigma-aldrich.com/enzymeexplorer.

Biochem/physiol Actions

Preferential cleavage: hydrophobic and aromatic residues in P1 and P1′ postitions. Cleaves Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe and Phe-Tyr bonds in the β chain of insulin

Unit Definition

One unit will produce a ΔA280 of 0.001 per min at pH2.0 at 37° C, measured as TCA-soluble products using hemoglobin as substrate. (Final volume = 16mL. Light path = 1cm.)

Analysis Note

Optimum pH is 2-4. Active in 4 M urea and 3 M guanidine HCl. Stable at 60 °C. Pepsin is irreversibly inactivated at pH 8.0 - 8.5.
Protein determined by E1%/280

Other Notes

View more information on pepsin at www.sigma-aldrich.com/enzymeexplorer.

inhibitor

Product No.
Description
Pricing

pictograms

Health hazardExclamation mark

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


  • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number

    Don't see the Right Version?

    If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

    Already Own This Product?

    Find documentation for the products that you have recently purchased in the Document Library.

    Visit the Document Library

    Customers Also Viewed

    J A Green et al.
    Reviews of reproduction, 3(1), 62-69 (1998-03-24)
    The pregnancy-associated glycoproteins (PAGs) were first described as placental antigens of cattle that were also present in the blood serum of the mother after implantation. Molecular cloning studies have shown that they are members of the aspartic proteinase gene family
    Joseph S Fruton
    The Quarterly review of biology, 77(2), 127-147 (2002-07-02)
    Studies on gastric digestion during 1820-1840 led to the discovery of pepsin as the agent which, in the presence of stomach acid, causes the dissolution of nutrients such as meat or coagulated egg white. Soon afterward it was shown that
    J Tack
    Alimentary pharmacology & therapeutics, 22 Suppl 1, 48-54 (2005-07-27)
    Gastro-oesophageal reflux disease is defined as the presence of symptoms or lesions that can be attributed to the reflux of gastric contents into the oesophagus. Aspiration and prolonged monitoring studies in humans have shown that reflux of gastric contents is
    B I Hirschowitz
    The Yale journal of biology and medicine, 72(2-3), 133-143 (2000-04-26)
    Esophagitis results from excessive exposure of the esophagus to gastric juice through an ineffective or dysfunctional lower esophageal sphincter mechanism. A possible role of pepsin in damaging the esophageal mucosa with consequent esophagitis may be examined directly by testing pepsin
    Autonomic control of secretion of gastric acid and pepsin.
    K Bech
    Journal of autonomic pharmacology, 9(6), 419-428 (1989-12-01)

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service